answersLogoWhite

0


Want this question answered?

Be notified when an answer is posted

Add your answer:

Earn +20 pts
Q: What is the function of quadrant in plate streaking technique?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Related questions

What is the technique you used in purify the bacterial culture on a plate?

Selective media, combined with quadrant streaking to dilute the sample into individual colonies.


Why can't we talk when streaking LB Agar plates?

Because when u talk while streaking some bacteria could come from your mouth and get on the plate from spit or somehow. There fore talking is not a very good aseptic technique.


Advantages of pour plate method over other methods of bacterial colony?

The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.


What is the differences between a streak plate technique and a serial dilution technique?

what is serial dilution and spread plate technique


What is crowded plate technique?

Williston et.al and waksman et.al discovered the crowdwed plate technique.


The white powder left behind by a mineral on a white tile?

When you rub a mineral on a tile it is called streaking a mineral. The line that is left is called the streak, and the tile itself is called a streaking plate.


What is strip plate technique?

i'ts where you strip in front of a plate


Why does the streaking method you used to inoculate you plates result in isolated colonies?

In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.


What is the significance of using a 'dry and hard' agar plate in streak plating?

Why is it impotant to use dry and hard agar for streaking


What are the steps in properly streaking a plate?

When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.


Pour plate and spread plate?

A pour plate is an alternative method for using agar plates to obtain isolated colonies. Pour plates are used when it is necessary to know the number of organisms present per unit volume of specimen or other sample. When a specific aliquot is placed in the Petri dish, a count of the colonies that grow after incubation reveals their concentration in the original sample. Pour plates are used commonly in the bacteriologic examination of milk, or could also be used to determine whether sufficient bacterial numbers are present in urine samples to signify the patient has a urinary tract infection. The number of bacteria in solution can be readily quantified by using the spread plate technique. In this technique, the sample is appropriately diluted and a small aliquot transferred to an agar plate. The bacteria are then distributed evenly over the surface by a special streaking technique. After colonies are grown, they are counted and the number of bacteria in the original sample calculated. Streaking in this technique is done using a bent glass rod. 0.1 mL of bacterial suspension is placed in the center of the plate using a sterile pipet. The glass rod is sterilized by first dipping it into a 70% alcohol solution and then passing it quickly through the Bunsen burner flame. The burning alcohol sterilizes the rod at a cooler temperature than holding the rod in the burner flame thus reducing the chance of you burning your fingers. When all the alcohol has burned off and the rod has air-cooled, streak the rod back and forth across the plate working up and down several times. Unlike streaking for isolation, you want to backtrack many times in order to distribute the bacteria as evenly as possible. Turn the plate 90 degrees and repeat the side to side, up and down streaking. Turn the plate 45 degrees and streak a third time. Do not sterilize the glass rod between plate turnings. Cover the plate and wait several minutes before turning it upside down for incubation. This will allow the broth to soak into the plate so the bacteria won't drip onto the plate lid.


A piece of string 25cm long goes exactly once around the edge of a plate in the form of a quadrant Calculate the area of the quadrant?

Presumably the plate is completely flat. The circumference will be 4*25 = 100 cm. First find the radius of the plate by rearranging the formula: 2*pi*radius = 100cm radius = 100/2*pi = 15.91549431 cm. Now use the formula pi*radius2 to find the area of the plate, and then divide your answer by 4 to give you the area of the quadrant. pi*15.915494312 = 795.7747155/4 = 198.9436789cm2. Therefore, the area of the quadrant is 199 cm2 correct to three significant figures.