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There are many objects that are difficult to see with a microscope. The purpose of the contrast is to make it easier to see specimens that are transparent and unstained.
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What concept of microscopy deals with the ability of the specimen to stand out against its background?
Contrast
What is the difference between phase-contrast microscopy and bright-field microscopy?
Phase-contrast microscopy is the observation of internal structures of living microbes where as bright field microscopy is the observation of killed stained specimens and naturally colored live ones.
Which of the following would be best observed for a bacterial cell using phase contrast microscopy?
Motility
Which works best with phase contrast microscopy wet mount or hang drop?
wet mount slide
Which type of microscopy is used for colorless specimens?
Transmission electron microscopy
The two most common methods for visualizing live cells are phase-contrast microscopy and differential interference contrast microscopy?
perhaps
What concept of microscopy deals with the ability of the specimen to stand out against its background?
Contrast
What is the difference between phase-contrast microscopy and bright-field microscopy?
Phase-contrast microscopy is the observation of internal structures of living microbes where as bright field microscopy is the observation of killed stained specimens and naturally colored live ones.
Which of the following would be best observed for a bacterial cell using phase contrast microscopy?
Motility
Where would phase contrast microscopy would improve image quality?
whenever you're looking at a wet mount you want to use phase contrast
Which works best with phase contrast microscopy wet mount or hang drop?
wet mount slide
What type of slide works best with phase-contrast microscopy?
Hanging drop can be observed for longer periods of time without drying out.
Why is staining uranium important?
Uranyl acetate and uranyl formiate increase the contrast in electron microscopy of viruses, cells, biological samples, protein molecules, etc.
What is a confocal microscopes?
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. cited works: wikipedia
What is confocal microscope?
Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. cited works: wikipedia
Introduction in basic techniques in microscopy?
Introduction to basic techniques in microscopy involves light microscopy, laser scanning, types of dyes, the cell, electron microscopy, differential interface microscopy, histological stains and histochemical stains.
What is Dark field microscopy?
Dark field microscopy (dark ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen (i.e. where there is no specimen to scatter the beam) is generally dark.
