Dry heat = 1-2hrs @ 160-170 degrees Celsius
Moist heat = 15min. @ 121 degrees Celsius
endothermic means it is absorbing heat. the heat within the organism would be warmer than the surrounding environment over time.
Mechanism of killing by dry heatDry heat kills the organisms by destructive oxidation of essential cell constituentsKilling of the most resistant spores by dry heat requires a temperature of about 160 °C for 60 minutesDry heat is employed for glassware; syringes, metal instruments and paper wrapped goods, which are not spoiled by high temperatures.It is also used for anhydrous fats, oils and powders that are impermeable to moisture.Mechanism of killing by moist heatMoist heat kills the organisms by coagulating and denaturing their enzymes and structural protein.Sterilization by moist heat of the most resistant spores generally requires 121 °C for 15-30 minutes.Moist heat is used for the sterilization of culture media, and all other materials through which steam can penetrateMoist heat is more effective than dry heatSterilization can be done at lower temperatures in a given time at a shorter duration at the same temperature.upon heating wet proteins release free -sh groups and form smaller peptide chains which in turn form new complexes different from the orignal protein molecules
The pasteurization is carried out for a prolonged time at around 170°F and is supposed to kill only heat susceptible organisms and their spores, while sterilization works at a temperature of 250°F and is supposed to kill all organisms.
Fo = Equivalent sterilization time Equivalent sterilization time at 121.1°C is 1 min Ratna Kishore Katam Microbiologist
It works 99.6% of the time
power rating is the rate of moving energy their relationship in the amount of heat it produce it divided by time to move that much energy
Since different objects have different heat conductivity and different temperatures and heat capacities, the time can vary without limit.
Water has a higher heat capacity than air. In other words, air is a very poor conductor of heat while water is a very efficient conductor of heat.
Oceans make temperatures less extreme, was water tends to take a long time to heat up and cool down.
Heat tape is usually used for electrical parts or pipes that might be exposed to high temperatures. Most contractors or do-it-yourself type people use it all the time.
The degree of sterilization is expressed by multiples of decimal reduction time D denoting time needed to reduce the initial number N_0 to one tenth (10^{-1}) of the original value.
Steam sterilization uses much less energy and time than dry heat methods. In order to kill spores a dry heat sterilizer needs to reach and maintain 170°C for one hour. An autoclave only needs to reach 121°C for 15 minutes.