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The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
It solubalize lipids and protiens to remove them from DNA
This step is used to danature DNA (separate two strands) by NaOH: Denature the DNA by adding freshly prepared NaOH (3 M) to a final concentration of 0.3 M. Incubate at 42°C for 30 min.
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
It plays a role.
roll of Na CL in DNA extraction
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
lyses cells
Its purpose is to isolate DNA from a protein mixture.
To give the solution buffering capacity.
stabilization of phenol against oxidation
This question gave me cancer.
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation
Papain contains protease which is an enzyme that is responsible for removing the proteins in DNA. Which would be the histones, in which DNA is wrapped around
STE stabilize the lysosom's membrane stop the proteinase liberation