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Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
Role of te in dna extraction?
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
What is the difference between an amino acid and RNA?
RNA is a polymer of ribonucleic acids. Amino acids are the monomers of proteins.
During transcription the genetic information is rewritten as a molecule of?
During transcription, the genetic information is rewritten as a molecule of
The two main types of flow of the genetic information are?
1.central dogma/unidirectional flow of information DNA to rna to proteins 2.central dogma reverse rna to DNA to rna to proteins e.g.in hiv
What is the function of formamide loading buffer?
Formamide loading buffer is used in nucleic acid gel electrophoresis to denature DNA or RNA samples before they are loaded onto the gel. It helps separate double-stranded DNA into single strands by disrupting hydrogen bonds, allowing for accurate size separation during electrophoresis. Additionally, the formamide loading buffer contains a tracking dye that helps monitor the progress of the electrophoresis run.
Why use formaldehyde in northern blotting for preparation of gel?
The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus preventing RNA degradation by high temperatures.And also The RNA samples are most commonly separated on agarose gels containing formaldehyde as a denaturing agent for the RNA to limit secondary structure.
What is formamide?
It's a feedstock for quite a few products, and it's made in one of three ways: reacting formic acid with ammonia then heating it; reacting carbon monoxide and ammonia; or reacting carbon monoxide and methanol to get methyl formate then performing an aminolysis reaction to get formamide and methanol (which you then react with more carbon monoxide to get more methyl formate...)
What is the role enzymes play in the procces of gel electrophoresis?
Gel electrophoresis is an analytical method used for the separation of DNA, RNA or proteins based on size.Enzymes are not requires to carry out this process
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
How can RNA be separated and visualized using acrylamide gel electrophoresis?
RNA can be separated and visualized using acrylamide gel electrophoresis by first denaturing the RNA samples, then loading them onto the gel and applying an electric current. The RNA molecules will move through the gel based on their size, with smaller molecules moving faster. After electrophoresis, the gel can be stained with a dye that binds to RNA, allowing the bands to be visualized under UV light.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
Why is it difficult to separate large amounts of RNA and DNA using gel electrophoresis?
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What is the gel used for in gel electrophoresis?
The gel used in gel electrophoresis is a porous material that helps separate DNA, RNA, or proteins based on their size and charge when an electric current is applied.
What are the pros and cons of gel electrophoresis?
Pros: The detection of DNA, RNA and proteins can be done using gel electrophoresis. Gel electrophoresis does not require a large amount of starting material. Cons: difficult to extract samples for further analysis. Harmful materials.
Difference between southern and northern blotting?
Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis
What is a gel electrophoresis is not used for?
Gel electrophoresis is not typically used for determining the function of proteins or for studying protein-protein interactions. It is primarily used to separate and analyze DNA, RNA, or proteins based on their size and charge.
