What is the syllabus for biochemistry net exam?

Answer 1

BIOCHEMISTRY-I

Amino acid structure & Nomenclature, individual properties and tests, Primary structure: peptide bonds, Amino acid titration, Isoelectric point Determination, Protein sequencing, Reagents and Enzymes involved, Secondary structure of Proteins, Dihedral angles, Ramchandran plot for PolyGly and PolyAla, ?-helix, ?.sheet, ? helix, 310 helix, Poly Pro I and Poly Pro II, Super secondary structure, Tertiary structure --Collagen, Keratin, Silk fibroin, Elastin. Macro and micro nutrients- related diseases. Vitamins and their chemistry Biochemical properties

Nomenclature, properties of enzyme Enzyme Kinetics:Michalis mentan equation turnover number, specific activity, Ks, Km, Determination, Line weaver burk plot. Edie Hofsteeplot, Hansplot. Inhibition Competitive, Uncompetitive and Non Competitive.

Globular Proteins: Myoglobin, Hemoglobin Allostery: Hill Plot and hill equation, PFK-1, ATCase, Receptor ligand kinetics, Scatchard plot, Negative & positive Copper activity, Studying receptor ligand kinetics, Protein Folding related diseases, Prions, ? CJD, Scrapie, HD, and others.

Techniques: Protein solubility techniques, Purity determination, Absorption Spectroscopy, Fluroscence Spectroscopy, IR-Spectroscopy. Raman Spectroscopy, Mass Spectroscopy, Centrifugation techniques, electrophoresis techniques. ESR, NMR, X-ray differection, and other relevant Techniques for Biochemical and Molecular Analysis.

BIOCHEMISTRY-II

Major metabolic pathways and their regulation

Cabohydrate Structure of mono, di and polysaccharides and their chemical reactions carbohydrate metabolism- glycolysis, gluconeogenesis, HMP, glycogen metabolism, TCA, oxidative phosphorylation, Regulation of glucose metabolism . Diabetes case studies. GAGs. Glycoproteins. oxidative phosphorylation

PROTEIN METABOLISM and urea cycle, heme and chlorophyll biosynthesis, protein related diseases.

L1PIDS Digestion, Absorption. Secretion. Transport through blood.Lipid biosynthesis, oxidation. Specialized fatty acids, ketone bodies. Phosphofipid metabolism , Glycolipid metabolism,. Cholesterol and steroid metabolism