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Nobody knows!: - Only the Absorption on ALL'visible' Wavelength together (= Visible Spectrum) determine the color of what the Cuvette is containing!

Of course, that there are also 'Colors' outside the human "'Visibility'", and that also the Lambdamax is only an absolute Strongman to determine any Color!

(Even if all Universities tell it the other way!)

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Why DNA absorb uv at 260 wavw length?

DNA absorbs UV light at 260 nm due to the presence of nitrogen-containing aromatic compounds called nucleic acid bases, such as adenine, guanine, cytosine, and thymine. These bases have conjugated double bonds that absorb light at this specific wavelength, allowing scientists to quantify DNA concentration through UV absorption.


What wavelength of uv light causes thymine dimmers?

Ultraviolet light with a wavelength of around 260 nm causes thymine dimers to form in DNA. Thymine dimers are the result of two adjacent thymine bases on the DNA strand becoming covalently bonded after exposure to UV light.


Why spectrophotometer is set in visible range 600nm while measuring bacterial turbidity?

When measuring light scattering it is important to consider the wavelength of light used a bacterial culture. Microorganisms may contain numerous macromolecules that will absorb light, including DNA (254 nm), proteins (280 nm), cytochromes (400-500 nm), and possible cell pigments. When measuring bacteria by light scattering it is best to pick a wavelength where absorption is at a minimum and for most bacterial cultures wavelengths around 600 nm are a good choice. However, the exact wavelength chosen is species specific. It is best to pick a wavelength where absorption is at a minimum.


What is the wavelength of UV light in laminar air flow bench?

UV light iddivided in to three. UV-C wave length from 100nm to 280nm. UV-B 280nm to 315nm and UV-A 315nm to 400nm. The wave length of 254nm (UV-C) is giving maximum germicidal activity and it is not too irritating.


What part of DNA absorbs uv light?

In suntan lotion it is avobenzone and/or octyl methoxycinnamate. Many organic molecules (natural and man-made polymers) absorb UV. DNA absorbs UV-B (which can cause damage) - as can the cornea of the eye! The cornea also absorbs UV-A but seems to not suffer much with it. Much of the sun's UV-A, UV_B and UV_C radiation is absorbed by the Ozone Layer when it enters the atmosphere.


Why DNA absorbs uv light at 260nm?

DNA absorbs UV light at 260nm due to the presence of nitrogenous bases (adenine, guanine, cytosine, thymine) that contain aromatic rings capable of absorbing UV light at this wavelength. This absorption allows for the quantification and analysis of DNA samples using UV-spectrophotometry.


Why does DNA absorb more light when it is converted into single strands?

in double stranded dna, the sugar phosphate backbone forms the the outside of the double helix and the information is found within (the complementary base pairs). When you heat up double stranded DNA, the DNA begins to unwind. The increases in temperature breaks the hydrogen bonds between the complementary base pairs and they now have become "unstacked" / exposed = (single stranded DNA). This allows the the single stranded DNA to absorb more light.


How does photolyase use light energy to repair DNA?

Photolyase works in conjunction with two co-factors. The first co-factor (either deazaflavin or methenyltetrahydrofolate) absorb a photon of light or a specific wavelength (in the blue spectrum) and passes this energy to FADH- which then acts as an electron donor, transferring a single electron to the pyramidine photodimer, and in doing so allows the bond between them to be broken.


Which requires a shorter wavelength to break- one of the bonds in the molecule Ozone or Oxygen?

Ozone requires a shorter wavelength to break one of its bonds compared to oxygen. This is because ozone (O3) has a weaker bond due to having three oxygen atoms connected, which makes it easier to break compared to the double bond in diatomic oxygen (O2).


When in a cells DNA content at its maximum?

A cell's DNA content is at its maximum during the S phase of the cell cycle when DNA replication occurs. At the end of S phase, each chromosome has two sister chromatids, doubling the amount of DNA in the cell compared to G1 phase.


What is the maximum DNA storage capacity of a single cell?

The maximum DNA storage capacity of a single cell is around 6.4 billion base pairs.


Why does cytochrome c absorb uv and visible light?

Cytochrome c contains heme groups which can absorb UV and visible light due to the presence of conjugated double bonds in the heme structure. When light is absorbed, electrons in the heme group are excited to higher energy levels, leading to characteristic absorption peaks in the UV-Vis spectrum.