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What is the formula for void ratio?
Void ratio (e) is typically defined as the ratio of the volume of voids to the volume of solids in a soil sample. The formula for void ratio is e = Vv/Vs, where Vv is the volume of voids and Vs is the volume of solids in the soil sample.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is hplc column volume?
Since an HPLC column is a cylinder, the simplest estimate for the column volume is the equation V=L*pi*r2, where L = length of column (typically 50-250 mm, or 5-25 cm), and r=radius of the column, where typical internal diameters of HPLC columns are 2.1 mm, 3 mm, 4 mm, and 4.6 mm. For example, suppose you have a column that is 25 cm long by 4.6 mm internal diameter (ID). Since the ID is in mm, you first convert to cm, then divide by 2 to get 0.23 cm radius. The column volume equation then is: V = 25 * pi * (0.23)2 = 25 * pi * 0.0529 = 1.3225 * pi = 4.15 cm3 From there, you can convert cm3 to mL directly, so your column has a volume of 4.15 mL. However, you must also allow for the relative porosity of the packing material in your column, which is harder to measure. Typically, an unretained analyte will be injected through the column at a known flow rate, and the time it takes for the analyte to exit the column is used to determine a better approximation of column volume. In the case of using an unretained analyte (which in reversed-phase HPLC, the analyte might be Uracil), using the same 25 cm by 4.6 mm column above and a 1 mL/min flow rate, suppose the analyte elutes from the column at 3.2 minutes. The column volume would then be 3.2 minutes * 1.0 mL/min = 3.2 mL, which does not agree with the calculated column volume. This is due to the fact that the particles in the column take up some of the volume of the column, so the total column volume is reduced by the amount of space they take up.
What is void volume in chromatography?
bed volume= pie*r square*h where, pie=3.14 r=radius of column h=height of column it is the total volume of column packed with the gel.
Can melamine detect by HPLC?
Yes, melamine can be detected by HPLC (High Performance Liquid Chromatography). HPLC is a common analytical technique used to separate and quantify compounds in a mixture, including melamine. Detection methods such as UV-Vis spectroscopy or mass spectrometry can be used in conjunction with HPLC to identify and quantify melamine accurately.
What is mean by delay volume in HPLC analysis?
Delay volume in HPLC analysis refers to the volume of liquid in the system that is not actively participating in the separation process. It includes the volume of tubing, fittings, and the void volume of the column. Minimizing the delay volume is important for maintaining good chromatographic resolution and reducing analysis time.
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What is void rate?
Void ratio is defined as the ratio of the volume of the void space compared to the volume of the solid particles.
How do you determine column void volume?
Ideally, the void volume should be 40% of the total column volume.
How do you calculate concentration from peak area in HPLC analysis?
To calculate concentration from peak area in HPLC analysis, you can use the formula: Concentration Peak Area / (Slope x Injection Volume). The peak area is obtained from the chromatogram, the slope is the calibration curve slope, and the injection volume is the volume of the sample injected into the HPLC system.
What is the systm volume and what is the dead volume and what is the wide volume and what is the delay volme in HPLC?
system volume is amount of mobile phase travel from reservior to tip of the colomn . dead volume is the amount of mobile phase present in colomn. delay volume is amount of mobile phase used in at the time of injection and the time of detection of chromatogram.
How can you calculate the volume of void space of sand?
What fraction of the soil volume is void space
What is dead volum in hplc?
Dead volume in HPLC refers to the volume in the system that is not actively involved in the separation process. It includes the volume of tubing, fittings, and detector cell that the mobile phase passes through without interacting with the stationary phase or analytes. Dead volume can lead to band broadening and decreased resolution in chromatographic separations.
What is the formula for void ratio?
Void ratio (e) is typically defined as the ratio of the volume of voids to the volume of solids in a soil sample. The formula for void ratio is e = Vv/Vs, where Vv is the volume of voids and Vs is the volume of solids in the soil sample.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
