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Total volume of mobile phase in a fully wet packed column- the space between the particles of the stationary phase plus the volume within the particles
Wiki User
β 14y ago
Wiki User
β 12y agoThe time required to elute unretained peak from column is known as void time
Wiki User
β 12y agovoid time is the time spent by the solute molecules traveling through the chromatographic column with out spending any time in the stationary phase.
ζΊδ»ͺ εΎ
The time for an unretained solute to reach the detector from the point of injection is called the column void time or dead time or hold-up time.
Sophia Wambui
This is the time taken to elute non retained
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What is the formula for void ratio?
Volume of voids divided by Volume of solids
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
What is void volume in chromatography?
bed volume= pie*r square*h where, pie=3.14 r=radius of column h=height of column it is the total volume of column packed with the gel.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
What is dead volume HPLC?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What is dead volum in hplc?
The dead volume in HPLC is 137.45. The dead volume in science is used in retention measurements and also in thermodynamic studies and the abbreviation HPLC stands for High Pressure Liquid Chromatography.
What is void rate?
Void ratio is defined as the ratio of the volume of the void space compared to the volume of the solid particles.
How do you determine column void volume?
Ideally, the void volume should be 40% of the total column volume.
How can you calculate the volume of void space of sand?
What fraction of the soil volume is void space
What is mean by delay volume in HPLC analysis?
how long time gradient reach column.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What is the formula for void ratio?
Volume of voids divided by Volume of solids
Which one is advanced gc or hplc?
HPLC is advanced
What is the systm volume and what is the dead volume and what is the wide volume and what is the delay volme in HPLC?
system volume is amount of mobile phase travel from reservior to tip of the colomn . dead volume is the amount of mobile phase present in colomn. delay volume is amount of mobile phase used in at the time of injection and the time of detection of chromatogram.
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
