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Temporal isolation-species mating at different times of the year.

Behavioral isolation-mating behaviors in two groups of isolated populations change, thus making individuals from the two groups not attracted to each other.

Pollinator isolation-some closely related plant species have flowers that attract different pollinators, preventing the two species from inbreeding.

Mechanical isolation-when reproductive organs in closely related species change drastically, making it impossible for one species to mate with another.

Gametic isolation-when incompatibilities between the sperm and eggs of closely related species keep them from fusing.

Hybrid inviability or sterility-when the egg gets fertilized, the resulting offspring may not live (inviable) or it is sterile.

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