0
in pcr technique we take original dna first heat it to separate to strands in thermocycler then add rna primer after the formation of about 10 sequences on both parental strands add dna polymerase to construct further
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
How do you use watch glass chemistry?
- as a support to weigh samples of solid reagents- to cover a Berzelius beaker
Can chemicals and reagents go bad?
Yes only some... It depends
What is the function of tris hcl in PCR buffer?
IT act as a buffering agent to maintain the PH of a PCR
Why are Grignard reagents synthesized in an ether medium?
Grignard reagents are stable in ether medium. Ether generally forms an adduct with Grignard reagents. In addition, if solvents containing acidic hydrogens are present, Grignard reagents are decomposed to the corresponding hydrocarbon.
What substances do you have at the beginning of a chemical reaction?
The substances you have at the beginning of a chemical reaction are the reactants or the reagents.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
Why do you use a negative control in PCR?
to check is there any contamination in pcr products
Use of water in pcr?
enzyme cofactor
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What are the pros and cons of RT-PCR?
There are several advantages of using real-time PCR over other methods. Real-time PCR assays are thousand folds more sensitive than RNase protection assays or dot blot hybridization. It allows you to quite precisely calculate and compare of the amount of template in each cycle, instead of determining the amount of product at the end of the reaction. Quantitative RT-PCR is commonly used for clinical applications. For example, you could use this method to quantify the amount of HIV RNA particles per ml of blood plasma in a patient who is undergoing treatment with antiviral drugs to see if it's working or not. The main problem with real-time PCR is that it requires specialised thermal cyclers (PCR machine) with fluorescence monitors and its reagents are quite expensive.
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
What is the use of maintaining a certain pH in pcr?
PCR is an enzymatically guided process. In optimum pH the enzyme will work best.
What do physician use to screen a genetic disorder?
PCR
What is a method of making many copies of DNA?
Polymerase chain reaction
What equipment is use for collection and detection of biological agents?
PCR
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
What is the purpose of mgcl in pcr?
The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.
