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eyepiece lens, arm, base, tube, illuminator, objective lenses, diaphragm, and condenser lens

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Daryl Kemmer

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Q: What two parts of the microscope are used to move objective lens?
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Explain how focussing occurs in a microscope?

To focus the image, you have to move the object you are studying closer to or farther from the objective lens.


What is the lens that is closest to the eye in a light microscope?

On a microscope with the usual 3-lens turret it is usual to use the objective lens with the lowest magnification to first examine your specimen. This gives a wider overall view of the subject, and will allow you to choose the particular detail that best suits your study. You may then move on to a higher magnification, if necessary, to study finer detail. If you started with the highest magnification, your fine focus will be uncertain, and you risk the front of the objective lens coming into contact with the sample slide. This could damage your specimen, and may damage the front of the lens.


Why should you always move the objective lens away from the slide when you're focusing a microscope?

If you move closer while looking thru the lens, there is a danger of cracking the slide, especially using coarse adjustment.


Which lens should you use to first look at a slide on a microscope?

The lens that you should use to first look at a slide on a microscope is a low power lens then move on to a higher power for more clarity.


Which lens on a microscope should you use first to look at a slide?

The lens that you should use to first look at a slide on a microscope is a low power lens then move on to a higher power for more clarity.


What are the differences between a microscope and a hand lens?

When viewing a specimen through a hand lens, you can see in slightly greater detail than your naked eye, but you may not be able to see micro organisms. With a microscope, you can see things in MUCH greater detail, and you can see micro organisms. With a hand lens, you cannot change the power of magnification, while with a microscope, you can change the power of the magnification. With a hand lens, only one lens is used to magnify an object, while a compound microscope uses two lenses (one in objective lens, one in eyepiece). With a hand lens, you can view a specimen from far away, while an objective lens has to be fairly close to a specimen in order to view it clearly. With a hand lens, you can simply move your hand forward, or backward, to focus on an object. With a microscope, a very complex mechanism is used in order to portray some sort of depth while viewing. This is adjusted by using a coarse, and a fine adjustment knob.


How to use a microscope?

1. When moving your microscope, always carry it with both hands (Figure 1, below). Grasp the arm with one hand and place the other hand under the base for support.2. Turn the revolving nosepiece so that the lowest power objective lens is "clicked" into position (This is also the shortest objective lens).3. Your microscope slide should be prepared with a coverslip or cover glass over the specimen. This will help protect the objective lenses if they touch the slide. Place the microscope slide on the stage and fasten it with the stage clips. You can push down on the back end of the stage clip to open it.4. Look at the objective lens and the stage from the side (Figure 2) and turn the coarse focus knob so that the objective lens moves downward (or the stage, if it moves, goes upward). Move it as far as it will go without touching the slide!5. Now, look through the eyepiece and adjust the illuminator (or mirror) and diaphragm (Figure 3) for the greatest amount of light.6. Slowly turn the coarse adjustment so that the objective lens goes up (away from the slide). Continue until the image comes into focus. Use the fine adjustment, if available, for fine focusing. If you have a microscope with a moving stage, then turn the coarse knob so the stage moves downward or away from the objective lens.7. Move the microscope slide around so that the image is in the center of the field of view and readjust the mirror, illuminator or diaphragm for the clearest image.8. Now, you should be able to change to the next objective lenses with only minimal use of the focusing adjustment. Use the fine adjustment, if available. If you cannot focus on your specimen, repeat steps 4 through 7 with the higher power objective lens in place. Do not allow the objective lens to touch the slide!9. The proper way to use a monocular microscope is to look through the eyepiece with one eye and keep the other eye open (this helps avoid eye strain). If you have to close one eye when looking into the microscope, it's ok. Remember, everything is upside down and backwards. When you move the slide to the right, the image goes to the left!10. Do not touch the glass part of the lenses with your fingers. Use only special lens paper to clean the lenses.11. When finished, raise the tube (or lower the stage), click the low power lens into position and remove the slide.12. Always keep your microscope covered when not in use. Dust is the number 1 enemy!


What are the parts and meaning of mechanical microscope?

The Mechanical Parts are the objective lenses, Fine Adjustment, Coarse Adjustment, some of the microscopes can move the stage, and some microscopes can move the adjustable mirror... Hope I can HELP!


What is the function of the revolving nosepiece in the microscope?

The nose piece holds the odjective lense, rotates, and notes the positive stops for each lens.


Why why should you always move object lens away from the slide when you're focusing a microscope?

If you move closer while looking thru the lens, there is a danger of cracking the slide, especially using coarse adjustment.


Why is it important to only use the fine adjustment knob when using the highest power objective lens?

When using high power, the lens is very close to the sample - and so you risk damaging the slide and/or lens if they touch - therefore you only want to move the platform a little bit at a time. It is very similar with an oil immersion - you don't want to damage the lens by allowing the sample to touch it.


How to describe the feature of the microscope that makes it possible to move among the objective lenses with only minor focusing?

Parfocal