the desired gene is inserted into the plasmid and the plasmid is returned to the bacterium by transformation.
Plasmids are sections of DNA that are independent of the main chromosome found in prokaryotes. It does not have any proteins other than the ones associated with replication of the plasmid.
Plasmids are circles of DNA. These have genes that can be transferred from one bacteria that has it to another. These genes can code for a protein that one cell normally doesn't code for. This done by a process called bacterial conjugation.
Yes, they are produced in the ribosomes of your cells.
Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
Recombinant DNA technology, DNA is inserted into bacteria, it can be used to make large quantities of the desired protein., and it had its origins in two related fields. the first, microbial genetics, studies mechanisms by which microorganisms inherit traits. the second, molecular biology, specially studies how genetic information is carried in molecules of DNA and how DNA directs the synthesis of protein. Are you going to Kirkwood?
Plasmids are cloned and start producing protein .
Plasmids are sections of DNA that are independent of the main chromosome found in prokaryotes. It does not have any proteins other than the ones associated with replication of the plasmid.
Plasmids are circles of DNA. These have genes that can be transferred from one bacteria that has it to another. These genes can code for a protein that one cell normally doesn't code for. This done by a process called bacterial conjugation.
Plasmids.
Yes, they are produced in the ribosomes of your cells.
Ribosomes produce proteins in the cell.
Yes, they produce proteins.Yes.
Protein
plants dont have the enzyme that folds the protein in the exact manner(3-D) structure so they dont produce protein
Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
Recombinant DNA technology, DNA is inserted into bacteria, it can be used to make large quantities of the desired protein., and it had its origins in two related fields. the first, microbial genetics, studies mechanisms by which microorganisms inherit traits. the second, molecular biology, specially studies how genetic information is carried in molecules of DNA and how DNA directs the synthesis of protein. Are you going to Kirkwood?
Yes, they do.