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Might add extra, but i really hope this helps!
scanning objective - for locating the specimen on the slide (= low power objective)
high power objective - magnifies the specimen to provide a detailed image
coarse adjustment - used to focus the image when using low power
diaphragm - used to adjust the amount of light passing through the specimen
revolving nosepiece - holds the three objective lenses - it can be rotated to change the objective in use
arm - holds the stage and the lens system - can be used to tilt the microscope (but not advised)
substaGe lamp - provides a uniform illumination (more reliable than daylight !!)
What else can I help you with?
Initial focusing of any slide is done under the?
The initial focusing of a microscope slide is done under the lowest magnification objective lens, known as the scanning objective lens. This allows for a wider field of view and easier location of specific structures or areas of interest on the slide.
Which objective scanning low power or high power allows you to observe the greatest number of cells within the field of view?
Scanning a specimen using a low-power objective allows you to observe the greatest number of cells within the field of view. Low-power objectives have a lower magnification but a wider field of view compared to high-power objectives, enabling a broader view of the specimen.
Why is it important to only use the fine adjustment knob when using the highest power objective lens?
When using high power, the lens is very close to the sample - and so you risk damaging the slide and/or lens if they touch - therefore you only want to move the platform a little bit at a time. It is very similar with an oil immersion - you don't want to damage the lens by allowing the sample to touch it.
How do you examine a prepared slide using a microscope?
To examine a prepared slide using a microscope, first place the slide on the stage and secure it with the stage clips. Start with the lowest magnification objective lens, adjust the focus using the coarse adjustment knob, then fine-tune the focus with the fine adjustment knob. Once the image is in focus, you can adjust the magnification by rotating the nosepiece to switch to a higher magnification lens for detailed observation.
Why should the fine adjustment knob be used only with the HPO?
The fine adjustment knob should be used only with the high-power objective (HPO) because it allows for precise focusing at high magnifications. Using the fine adjustment knob with lower-power objectives can result in the objective lens hitting the slide, potentially damaging both the lens and the slide.
Initial focusing of any slide is done under the?
The initial focusing of a microscope slide is done under the lowest magnification objective lens, known as the scanning objective lens. This allows for a wider field of view and easier location of specific structures or areas of interest on the slide.
When scanning 4 times objective is Used the total magnification will be?
When using a 4x objective lens on a microscope, the total magnification is calculated by multiplying the objective lens magnification by the eyepiece magnification. If the eyepiece (ocular) lens is typically 10x, the total magnification would be 4x (objective) × 10x (eyepiece) = 40x. Therefore, when scanning with a 4x objective, the total magnification will be 40x.
If you use the coarse-adjustment knob to focus on an object with the high-power objective what problems will you encounter why?
Using the coarse-adjustment knob with the high-power objective can result in the objective lens getting too close to the slide, potentially damaging the lens or slide. This can also lead to the objective lens hitting the slide, causing a loss of focus and potential damage to both the lens and the slide.
Why should you never use the coarse adjustment knob on high power for a microscope?
Using the coarse adjustment knob on high power can potentially damage the microscope or the slide being observed due to the high magnification and close proximity of the objective lens to the slide. It is better to make coarse adjustments on low power to avoid hitting the objective lens against the slide.
What are the change involved when view the prepared slide under microscope using different objective lens?
it has to do with the resolution of the image of the slide obtained...the larger the objective lens value is the greater the resolution it will provide, greater the resolution higher the details.
What are some of the best slide scanning services available?
Some of the best slide scanning services available include ScanCafe, DigMyPics, and ScanDigital. These services offer high-quality scanning with options for different resolutions and formats. They also provide restoration services for damaged slides and typically have quick turnaround times.
Why should you watch from the slide before you lower the objective of your microscope?
Movements viewed through the microscope are exaggerated quite a bit. As such, it's best to attempt to line up the object on the slide by looking at the slide directly, rather than through the objective.
Why is coarse adjustment knob not commonly used when focusing under high power objective?
The coarse adjustment knob moves the objective a lot while the fine adjustment knob moves it just a little, so using the coarse knob under high power can cause the objective to hit the slide and potentially damage the slide or the objective. The fine adjustment knob provides more precise control for focusing under high power, allowing for better clarity and accuracy.
Which objective scanning low power or high power allows you to observe the greatest number of cells within the field of view?
Scanning a specimen using a low-power objective allows you to observe the greatest number of cells within the field of view. Low-power objectives have a lower magnification but a wider field of view compared to high-power objectives, enabling a broader view of the specimen.
What are the advantages of using low power objective for initial viewing?
Low power objectives provide a wider field of view, making it easier to locate specimens and navigate the slide. They also allow for quicker scanning of the sample to get an overview before moving to higher magnifications. Additionally, using a lower magnification helps reduce the risk of accidentally damaging the lens or slide.
Why do you use on or slide to be examined with the oil immersion objective?
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
Why should you only use the fine adjustment knob when you are using high power?
The fine adjustment knob should be used with high power magnification because it allows for smaller, more precise movements of the objective lens. This helps to prevent damage to the slide and objective lens, as well as minimize the risk of crashing the objective lens into the slide.
