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It depends if you mean Reverse transcription PCR (RT-PCR) or real time PCR (qPCR) - (there are misnomers often used with real time being (RT)
Earliest article I could find on reverse transcription is
Coupled reverse transcription-polymerase chain reaction (RT-PCR) as a sensitive and rapid method for isozyme genotyping. Gene, 1990, 93, 271-275 published by Mocharla, H., Mocharla, R., Hodes, M. E.,
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