Want this question answered?
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
Bacterial cells.
No, bacterial cells have no complex organelles like the ER.
The 2 are bacterial cells and protists cells
Those circular DNA molecules are called Plasmids.widely used in Recombinent DNA Technology
Production of human insulin from bacterial cells.
I think you must rethink about your question, but still I am giving the answer as I can understand that you are asking about recombinant DNA technology where bacterial DNA is used as it is a cloning vector (plasmid). In recombinant DNA technology the particular sequence of DNA that we want to replicate or want to produce in huge number, is attached either with plasmid of bacteria or a DNA of bacteriophage and thus produce the recombinant or hybrid DNA which is copied each time when the bacteria or bacteriophage multiply. In this way the hybrid DNA will be transferred from parent cell to daughter cells.
Herbert Boyer and Stanley Cohen created the first recombinant DNA organism using recombinant DNA technology, or gene splicing, which allows the manipulation of DNA. They showed that the gene for a frog ribosomal RNA could be transferred and expressed in bacterial cells. Boyer and Cohen removed plasmids, small rings of DNA located in a cell's cytoplasm, from a cell. Using restriction enzymes, they cut the DNA at precise positions and then recombined the DNA strands in their own way using DNA ligase enzyme. They then inserted the altered DNA into E. coli bacteria. The bacterial cells could be made to produce specific proteins using gene splicing. This technology was a major breakthrough for genetic engineering. Their experiments dramatically demonstrated the potential impact of DNA recombinant engineering on medicine and pharmacology, industry and agriculture.
Bacterial cell wall is made of peptidoglycan(a polymer consisting of both sugars and amino acids). So, when the bacterial cells along with the recombinant DNA is subjected to high temperature and suddenly brought down to low temperature(almost immediately - after 2mins), the protein structure gets denatured. By this, there form pores large enough for the recombinant DNA to enter the cell and express the desired gene when required.
In bacteria, if the plasmid containing the foreign DNA manages to get inside a bacterial cell, this sequence ensures that it will be replicated. In Plant Cells, if transformation is successful the recombinant DNA is integrated into one of the chromosomes of the cell.
1.Cleaving DNA The source chromosomes is cut into fragments of DNA.2.Producing recombinant DNA The DNA fragments containing the desired gene are inserted into viral or bacterial DNA. The recombinant DNA is then allowed to infect the target cells.3.Cloning cells infected cells are allowed to reproduce.Growing a large number of identical cells from one cell is known as cloning.4.Screening target cells Targets cells that have received the particular gene of interest are isolated.
It's called a plasmid, but it can't be used for eukaryotic cells, only prokaryotic (bacteria). It's the basis of recombinant molecular biology.
no, bacterial cells do not have genetic material
Mammalian Cells
Bacterial cells are not eukaryotic cells, which means they do not have a nucleus. They do, however, have chromosome.
Bacterial cells.
No, bacterial cells have no complex organelles like the ER.