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Why you freeze the pellet during DNA isolation?
PEllet is freezed approximately at 4 degree celsius this is done because the enzymes remain inactive at this temperature and is activated only when needed so that it doesnt degrade the solution containing DNA.
What is use of ethanol in DNA isolation?
Ethanol is used to precipitate the DNA. I.e. to bring the DNA out of solution. Precipitated DNA is then spun down and re suspended in the appropriate buffer that is suitable for sample storage
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What is the principle involved in the isolation procedure?
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Role of sucrose in DNA isolation from human blood?
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
What is role of Cetyl trimethyl ammonium bromide in DNA isolation?
it act as as a cationic detergent for the isolation dna from the given sample
Role of phenol chloroform in DNA isolation?
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
Why 70 percent ethanol is used for DNA pellet washing?
In the process of DNA isolation, ethanol is used to preciputate the DNA or bring the DNA out of solution. Once precipitated, DNA appears as a white cottony mass that can be seen with the naked eye. This precipitated DNA is then spun down and re suspended in the appropriate buffer suitable for storage.
What are the function of sodium acetate in dna isolation?
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.
Role of chloroform in DNA isolation?
It solubilizes lipids and a lot of proteins to remove them from the DNA.
