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The Rf value of the xanthophyll is two thirds that of carotene because xanthophyll has an H bond with cellulose, which slows it down, and makes it less soluble in the solvent. Carotene is more soluble in 9:1 petroleum-ether acetone solution, which caused it to be carried higher and have a larger Rf value.

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Regine L. Ong

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Q: Why does carotene have the highest Rf value?
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