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pH is the measure of H+ ions. The more the concentration of H+ ions, the less the pH value and the more the concentration of OH- ions and lesser the concentration of H+ ions, the more is the pH value.

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Q: Why does pH of medium rise when urea is hydrolyzed?
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Can urea neutralise pH water?

Urea water solution is neutral.


What is the pH of urea?

Under 5,5.


What functional group is easily hydrolyzed?

An ester group is probably the most easily hydrolyzed functional group. Especially at physiologic pH.


Explain the function of phenol red in the urea broth medium?

phenol red act as the pH indicator. As the substrate urea is split into products, the presence of ammonia caused alkaline environment that cause the phenol red to turn to deep pink. Useful to test Proteus vulgaris.


How pH of culture be influenced by urea hydrolysis?

It will cause the culture to become more basic...an increase in pH


Is enterococcus faecalis positive or negative with the urea broth test?

It is urease positive .It possesses the enzymes urease which hydrolyses urea to form ammonia. As a result the pH of the medium used for detection of urease enzyme (Urea Broth) increases and shows bright pink colour, since phenol red is bright pink in alkaline conditions.


Why does the medium's pH need to be standardized at 5.8 while preparing the MS medium?

Because it is the pH of the Apoplast. Cells are happy in it.


What is the pH of filler?

It is a acidic medium . . .


Does the pH of the medium change during sterilization?

yes. The PH will changer We have to adjust the pH after sterilization by filtration


What does a color change in an acid-base titration mean?

according to change of PH of medium where the indicator color is changed according to PH of medium


Why is the pH of the medium important in edta titration?

why is the pH of the meadium important in EDTA titration


What will lowering the pH of a medium do to the angle of refraction of light?

Lowering the pH of a medium, such as a liquid, will not have a direct effect on the angle of refraction of light. The angle of refraction of light is primarily determined by the refractive index of the medium, which is not inherently influenced by pH changes. However, extreme changes in pH may induce changes in the optical properties of the medium, potentially leading to alterations in the refraction of light.