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The minimum resolvable separation distance of a light microscope depends on the wavelength of illumination and the numerical aperature. Because the electron beam has a far smaller wavelength than light used in light microscopy, it achieves far better resolution and it doesn't even involve the NE.

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Q: Why does resolution power not depend on numerical aperture in EM?
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What is the limit of resolution if numerical aperture of condenser is 1.25 and low power objective lense is 25?

You also need to know the wavelength :)


What happens to the image when you increase magnification?

In a light microscope when magnifiaction increases resolution decreases and the object will apear blurred. It can be removed by putting immersion oil on slides or object which increase the refractive index and cause to increase the numerical aperture which ultimately cause the better resolution as resolution power depends on numerical aperture of lens. The immersion oil used can be cedar oil.


Calculate the resolving power if the wavelength is 600 nm and the numerical apertures are 0 0.2 0.4 0.6 0.8 and 1.0?

Use the Equation, Resolving Power=lambda/2(Numerical Aperture). So, given the values for Numerical Aperture(NA): If NA=0, then R=0, NA=0.2, then R=1500, NA=0.4, then R=750, etc. Simply solve the equation substituting the provided Numerical Aperture (NA) values in.


How can you enhance the resolving power of microscope?

By using immersion oil


What does resolving power of a microscope depend on?

In a light microscope the resolution of the image it can project is limited by the distance each photon travels in its wavelength. Beneath this minimum distance, the "noise" of the photon's movement along its path overwhelms any resolution the light source may otherwise provide.


The wavelength of light used plus the numerical aperature governs?

To find resolution power of optical microscopes.


What is the relation between coupling efficiency and numerical aperture of optical fiber?

Coupling efficiency = NA2 such that if you have an initial power output P0 you get P=P0*NA2


Calculate the limit of resolution for the oil lens of your microscope Assume an average wavelength of 500nm?

Resolving power = 0.5x wavelength/ numerical aperture (n sin theta)n sin theta in most microscope have value = 1.2 and 1.4therefore:R. P. = 0.5x500nm/ 1.25 = 200nm = 0.2 microns.(conv. 1000nm = 1micron).


What do you mean by resolving power of telescope?

The "resolving power" of a telescope is a measure of the ability of a telescope to distinguish between two separate objects that appear to be very close together in the sky.


What factors determine the limit of resolution of a light microscope?

The limit of resolving power of a microscope is described by the Abbe criterion: d=wl/NA d being the minimal resolvable distance between two spots of the object wl being the wavelength of the light used NA being the numerical aperture of the microscope, which is equal to n*sin(a) with n being the refraction index of the immersion liquid between object and objective a being the aperture angle because sin(a) is always smaller than 1 and n cannot rise above 1.7, the maximal resolving power of a microscope is about d=wl/2 and thus only depends on the wavelength of the light used, which normally will be about 600 nm.


What does the term resolving power refer to?

The question is about the resolving power of optical instruments like telescope and microscope.It is the ability of the instrument to resolve the images of two points that are close to each other. If dθ is the angular separation, resolving power is given by the formulaR = 1/dθ = D/1.22 λ where Dis the aperture of the objective; λ is the wavelength of the light .


The numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25 you are supplied with a filter that selects a wavelength of 520nm what is the limit of resolution?

346.7nm's