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If the culture contains any unwanted organisms, it could skew the results of the biochemical test potentially producing false-positive or false-negative results of the test. If the culture contains any unwanted organisms, it could skew the results of the biochemical test potentially producing false-positive or false-negative results of the test.

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15y ago
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15y ago

If you attempt to identify a culture that is not pure(in other words, containing two or more different species of bacteria), then you may incorrectly identify the bacteria you intend to find. A pure culture ensures that only one type of bacteria is present and ensures one answer regardless of how many times you test it.

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15y ago

no one knows. if they did they woulda answered this question ages ago. who ever made this question must of lost it because they could have sat down for 3 seconds and straight looked it up on Google. and they have no excuse. whoever made it you know i am right. i am never wrong when it comes to craziness. so look it up on google or something. it will take 3 seconds. oh yeah i almost forgot on YouTube there is a conflict between this bunny and bob on the comments. it is about: Bob is a real person just about on every comment for a video and he wants us to paste him on every video so he can take over youtube. if we do so it will happen and it will be on the news. The bunny is a real person who does not want bob to take over. he says think about the children. he (the bunny) wants to keep the youtube how it is. paste the bunny on the videos also to save youtube. THANK YOU!

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13y ago

This is to ensure that only the correct bacteria to be grown in the medium is grown and there is no contamination.This is to prevent incorrect identification of bacteria as the medium is contaminated. Hence, pure cultures must be used.

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11y ago

Pure cultures are important in Microbiology for the following reasons:

  • Once purified, the isolated species can then be cultivated with the knowledge that only the desired microorganism is being grown.
  • A pure culture can be correctly identified for accurate studying and testing, and diagnosis in a clinical environment.
  • Testing/experimenting with a pure culture ensures that the same results can be achieved regardless of how many time the test is repeated.
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13y ago

To isolate specific microorganisms for study, classification, identification, genetics, production of certain organic substances, etc. If you need any ONE type of microorganism, you will need to perform aseptic techniques to aqcuire your pure culture.

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Q: Why is it essential to have pure culture for biochemical test?
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Why is pure culture necessary for enterotube tests but not for DNA probes?

For biochemical tests, such as the Enterotube, if the culture contains any unwanted organisms, it could distort the results of the biochemical test potentially producing false-positive or false-negative results of the test. You use the Enterotube test to find one unknown MO; therefore, if you don't use a pure culture, you don't know for sure what MO you identified with your results.On the other hand, when and MO is difficult to culture/grow you use a DNA probe. DNA probes bind directly to predefined nucleic acid sequences, which basically identifies the MO even when surrounded by other MO's. Therefore, a pure culture is unnecessary.


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