If the culture contains any unwanted organisms, it could skew the results of the biochemical test potentially producing false-positive or false-negative results of the test. If the culture contains any unwanted organisms, it could skew the results of the biochemical test potentially producing false-positive or false-negative results of the test.
If you attempt to identify a culture that is not pure(in other words, containing two or more different species of bacteria), then you may incorrectly identify the bacteria you intend to find. A pure culture ensures that only one type of bacteria is present and ensures one answer regardless of how many times you test it.
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This is to ensure that only the correct bacteria to be grown in the medium is grown and there is no contamination.This is to prevent incorrect identification of bacteria as the medium is contaminated. Hence, pure cultures must be used.
Pure cultures are important in Microbiology for the following reasons:
To isolate specific microorganisms for study, classification, identification, genetics, production of certain organic substances, etc. If you need any ONE type of microorganism, you will need to perform aseptic techniques to aqcuire your pure culture.
biurets test is the biochemical test for enzymesan enzyme is a protein and as the biochemical test for proteins is biurets,, its the same
Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.
To identify the pure culture the best method is to perform sub culturing and it can be done either by on another agar plate by streak plate method or by pour plate methodThe next is to perform staining which give the difference in morphology if the culture is not pure. But we should perform both the test and then should corelate them because none of them is individually 100% full proof
Try Bergey's Manual of Determinative Bacteriology
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
For biochemical tests, such as the Enterotube, if the culture contains any unwanted organisms, it could distort the results of the biochemical test potentially producing false-positive or false-negative results of the test. You use the Enterotube test to find one unknown MO; therefore, if you don't use a pure culture, you don't know for sure what MO you identified with your results.On the other hand, when and MO is difficult to culture/grow you use a DNA probe. DNA probes bind directly to predefined nucleic acid sequences, which basically identifies the MO even when surrounded by other MO's. Therefore, a pure culture is unnecessary.
biochemical test for p.gingivalis
the most commonly used biochemical test to identify lactobacillus is carbohydrate fermentation, this is the preliminary screening test and an essential test for the identification of lactobacilli
To determine accuracy
There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.
biurets test is the biochemical test for enzymesan enzyme is a protein and as the biochemical test for proteins is biurets,, its the same
You can't use a Biochemical test to ID a virus.
catalase
Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.
It should be negative.
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