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Capture and detection antibodies must recognise two non-overlapping apitopes in order to work. Once the detection antibody is bound, the capture antibody cannot obscure the epitope used by the detection antibody in any way, or the sandwich ELISA will not work. Hope that helps, I am also trying to answer a similar question and this is what i have found out so far, but not 100% sure that its right. Eve

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Q: Why is it important that the capture and detection antibodies recognize different epitopes in ELISA?
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