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To determine accuracy

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Q: Why must a pure culture be used in the Kirby-Bauer test?
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What is the reagent used to test Dnase test?

There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.


Did you get a pure culture in your subculture and how to know?

Ensuring that your culture is pure is very important to getting accurate results of your gram stain. One way to tell if your culture is pure is to test both the control and the experiment cultures. If you get a different result than the one you were expecting, you might have a corrupted culture. Making sure that you have several items of each is helpful, as well.


Why must the inoculating loop be cooled before touching a culture?

To avoid killing the test subjects before the test can begin.


Would it be useful to perform a gram stain on a mixed culture?

The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.


Why is pure culture necessary for enterotube tests but not for DNA probes?

For biochemical tests, such as the Enterotube, if the culture contains any unwanted organisms, it could distort the results of the biochemical test potentially producing false-positive or false-negative results of the test. You use the Enterotube test to find one unknown MO; therefore, if you don't use a pure culture, you don't know for sure what MO you identified with your results.On the other hand, when and MO is difficult to culture/grow you use a DNA probe. DNA probes bind directly to predefined nucleic acid sequences, which basically identifies the MO even when surrounded by other MO's. Therefore, a pure culture is unnecessary.


Why must the inoculating loop be cooled first before touching a culture?

To avoid killing the test subjects before the test can begin.


Can a Pure culture prepared from mixed broth or mixed agar slant?

To identify the pure culture the best method is to perform sub culturing and it can be done either by on another agar plate by streak plate method or by pour plate methodThe next is to perform staining which give the difference in morphology if the culture is not pure. But we should perform both the test and then should corelate them because none of them is individually 100% full proof


What is the pure text of a magnet?

The pure test of magnetism is repultion


What is a culture test?

A culture-fair test is test designed to be free of cultural bias, as far as possible, so that no one culture has an advantage over another.


Why is the DNase test only incubated for 24 hrs?

For the DNase test, 24 hours is sufficient to be able to examine the culture. The test result should be recorded after this amount of time has passed, and then it must be discarded.


Why is it essential to have pure culture for biochemical test?

If the culture contains any unwanted organisms, it could skew the results of the biochemical test potentially producing false-positive or false-negative results of the test. If the culture contains any unwanted organisms, it could skew the results of the biochemical test potentially producing false-positive or false-negative results of the test.


What is culture-fair test?

A culture-fair test is test designed to be free of cultural bias, as far as possible, so that no one culture has an advantage over another.