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if it wouldn't be above the level of solvent the spots will run off

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11y ago
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2mo ago

If the solvent level reaches the pencil line where the spots are, it can dissolve the compounds in the mixture, causing them to spread out and overlap. This can lead to inaccurate results and difficulties in analyzing the separated components on the chromatogram. It's important to maintain the separation of the compounds on the chromatography plate for clear identification.

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Q: Why must the level ofthe solvent not reach the pencil line where the spots are?
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Why must the level of the solvent not reach the pencil line where the spots are?

Allowing the solvent to reach the pencil line could oversaturate the spot, leading to uneven separation and inaccurate results. It may cause the compounds in the spot to spread out too much or merge with neighboring spots, making it difficult to accurately identify and analyze the components.


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