We use this principe when the annealing temperature of the two primers (reverse and forward) is different
to check is there any contamination in pcr products
enzyme cofactor
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.
It Inhibits the PCR reaction by chelating the magnesium ions.
The use of dNTP is PCR and multiplex PCR
to check is there any contamination in pcr products
enzyme cofactor
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
PCR is an enzymatically guided process. In optimum pH the enzyme will work best.
PCR
PCR
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.
U would say the score 1.
PCR