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Q: Why you are using methanol and chloroform in lipid extraction?
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What is successive extraction?

Successive extraction of any plant part involves sequential extraction ( e.g. employing soxhlet apparatus or maceration) using solvents with increasing polarity. Following each extraction, the marc (refuse matter that remains after extract has been filtered) will be dried before being extracted using solvent with higher polarity. For example, the following solvents can be used in successive order (lower to higher polarity): petroleum ether then chloroform then ethyl acetate, then methanol.


What is the Process of extraction of volatile oils?

1.Hydro-distillation: water distillation/Stam distillation. 2.Enfleurage: Extraction of delicate perfumes. 3.Eculle: mechanical extraction. 4.Solvent extraction: using lipid solvents.


If beeswax cholesterol and phosphatidylglycerol were dissolved in chloroform then subjected to thin layer chromatography on silica gel using a mixture of chloroform methanol water as the developing so?

Umm... what? Please, a little ENGLISH would be helpful. I don't speak alienated science.


How harmful is chloroform to man?

Chloroform using as a anesthetic. It may cause for the dath.


If you use chloroform spray for women?

Using chloroform spray for women is illegal. Chloroform spray is a carcinogenic. If inhaled, a person will pass out and become ill.


Low cost thinner using huge quantity methanol. have you any formula for manufacturing very low cost thinner by using methanol. which dissolved oil paint base and NC paint.?

s using methanol v can manufagture n c thinner


When did they stop using chloroform?

We used it in the past. We used it as a anesthetic.


How do you make Unconscious a human being by using which chemical spray?

by using halo-thane or chloroform


What is a sentence using the word lipid?

The lipid content of a shampoo determines if it will make your hair look shiny or greasy.


Can you make 2 sentences using the word lipid?

A lipid is made of chains of fatty acids. A lipidis a macromolecule.


What is the principle of DNA isolation using phenol chloroform method?

Phenol chloroform extraction is the oldest and still widely followed method for the isolation and extraction of DNA from plant and animal cells. The phenol, chloroform (and also isoamyl alchohol) are added in a specific ratio of 25: 24:1.Phenol: Phenol dissolves the organic impurities, like proteins etc.chloroform: Provides density to phenol so that it settles below water during phase separation.Isoamylalchohol: Used to prevent phosgene from reaction of chloroform with air.The Phenol:Chloroform:Isoamylalchohol (PCI) solution is added to the cell extract after removal removal of debris. After proper mixing, cetrifugation is done to separate the phases. Two phases are formed: The upper, the aqueous phase that contains DNA, the lower phase, that phenol phase, that contains organic impurities. Thus two phases are separated by a very clearly defined boundary of coagulated proteins.The aqueous phase is precipitated and then the DNA could be pelleted after rounds of purifications.


How many non-bonding electrons are in methanol?

This is a chemical element. You can find the how many electron in a single atom by using a periodic table.