Resolution is the ability of a lens to separate or distinguish between small objects that are close together. Much of the optical theory underlying microscope design was developed by the German physicist Ernst Abbe in the 1870s. The minimum distance (d) between two objects that reveals them as separate entities is given by the Abbe equation, in which lambda () is the wavelength of light used to illuminate the specimen and n sin theta is the numerical aperture (NA). d= .5 x lambda n sin theta
the resolving power of light microscope is 0.2 micrometr
The resolving power of the transmission electron microscope is 100 times better than the resolving power of the scanning electron microscope. The resolving power of the compound light microscope is 1000 times better than the resolving power of the unaided eye.
Resolving power is a function of numerical aperture of the objective lens use in the microscope and the wavelength of light . The formula for resolving power is given as , RP= wavelength / (2 x NA) where NA is the numerical aperture.
The resolving power of a microscope is a linear function of the wavelength - An optical microscope's wavelength is that of light, and the electron microscope's - that of vibrating electrons. As the electron microscope's wavelength is about 100,000 times smaller than that of light, we get a much better resolving power.
Resolving power of microscope is inversely related to the wavelength of the light used. So shorter the wavelength, greater the resolving power.
.2 um
A transmission electron microscope.
The magnifying power of a microscope is how big the microscope can make the image and how close it can make the image to the eye. Resolution or resolving power is how clear the microscope can focus the object and how much detail is provided in the magnification.
The wavelength of the electron.
Contrast increases, while resolving power decreases.
Resolving Power
0.2 micrometer