The ingredients for meat tenderizers is: salt, dextrose, bromelain, tricalcium phosphate, hydrogenated soybean and cottenseed oil.
According to "Yahoo Answers" meat tenderizers contain proteases which help that break down proteins, histones.
Today, we did a lab in our Biology class. In the lab we used oninons to extract DNA. Luckily, my group did really well this time - WITHOUT messing the labs (as we usually do). *clap clap - cheer*
Anyhow, we are also trying to answer our lab quesitons, and happened to stumble upon your question - or this quesiton rather as i don't know who posed the question.
Once again, refer to "Yahoo Answers" as it has a detailed answer to the question.
:)
Meat tenderizer does not in itself extract DNA. Tenderizer is an enzyme ( usually a bromeliad) which helps break down the proteins in the cell.
When the soap is added, the enzymes in the detergent will break down the lipids in the cell membrane like soap would do to a greasy pan. Also, in the meat tenderizer portion of the lab, the enzymes in the tenderizer will break down the proteins.
Calcium
It plays a role.
roll of Na CL in DNA extraction
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
lyses cells
Its purpose is to isolate DNA from a protein mixture.
To give the solution buffering capacity.
stabilization of phenol against oxidation
This question gave me cancer.
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation