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Urine testing

Inexpensive strip test that detect cotinine ( nicotine metabolite) for about 2-6 days/ cutoff around 200 ng/ml.

More sensitive Lab urine tests such as GCMS (cutoff 5 ng/ml ) and ion pair reversed-phase chromatography (cutoff 2 ng/ml) detect for about 7-12 days.

Hair TestingNicotine and cotinine can be Lab detected in hair via GC/MS, with a detection cutoff around 2 ng/ml (nicotine). Hair tests have a much longer detection window than fluid tests up to 90 days.

Saliva Testing

Cotinine can be detected by quick, inexpensive saliva tests. Some saliva tests are extremely sensitive, detecting cotinine at concentrations as low as 1 ng/ml. Nicotine and cotinine can be detected in saliva with more sensitive and expensive procedures, such as HPLC. The detection window for these procedures is around 7-10 days.

Blood Testing

Nicotine and cotinine are detectable in the blood with laboratory testing, for about 7-12 days.

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