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What are the disadvantages of paper chromatography over thin layer chromatography?
Wiki User
∙ 11y ago
Fear god and think about your death (the eternal life if you are a christian woe to you jesus is not god or the son of god if you are an atheist woe to you who is making you live now and if you are a muslim job well done may allah make your life easy for and have mercy on you.
Wiki User
∙ 11y ago
Wiki User
∙ 15y agoAs my understanding, after you had identified the fraction(s) which is desired, cut the part off and scrape the sorbent down into a test tubes/ beaker. Then dilute the fraction together with sorbent with diluents, filter tru filter paper. Evaporate off the solution that u obtain. (taken weight of tubes/ evaporating flask before (blank) and after evaporating, then yield of fraction can be obtained). Hope this can help you. have a nice day As my understanding, after you had identified the fraction(s) which is desired, cut the part off and scrape the sorbent down into a test tubes/ beaker. Then dilute the fraction together with sorbent with diluents, filter tru filter paper. Evaporate off the solution that u obtain. (taken weight of tubes/ evaporating flask before (blank) and after evaporating, then yield of fraction can be obtained). Hope this can help you. have a nice day
Wiki User
∙ 12y agoThin layer cromatography and paper chromatography relate to each in the following ways
1. Both have stationary phases and mobile phases
2. Both can be used to determine the retardation factor of a substance
3. Both are method of seperation
Wiki User
∙ 12y agowhats the comarative and the subjonctif of thin
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Disadvantages of using paper chromatography?
TLC has following advantages over paper chromatography: greater speed greater sensitivity for many substances than paper Small sample requirement Usually sharper preparation Different kind of reagents can be applied without damaging the plate
How is chromatography used to study plant pigment?
chromatography is basically a technique used for the separation of different components.... plant pigment consist of different components..... the sample is taken nd spotted over a chromatography paper..... nd den it is kept in suitable solvent to get separated...... different components travel different distance on chromatogram.... dis is how v cn use chromatography to study plant pigments...
Why is chromatography used in the police?
the police use chromatography to solve crimes, for example if they wanted to solve if a drug or a substance was illegal or legal they would use paper chromatograhy which would mean they would place the substance or a drug on paper dip it in a testing liquid which could be water for example and if the paper turns purple for example that liquid may be illegal or if that dot of the certain substance rises over 5cm for example it may be illegal so chromatography comes in very handy for the police and solving there crimes ! Hope this helped ;) xxx
Why is ink never used for drawing the baseline on the chromatography paper?
Depending on the ink and the solvent used for the chromatography, the ink might also migrate and be separated into its components or smear over the paper. With the graphite of a pencil, this will not happen.
What is meant by tailing a spot in Chromatography?
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
What are the Advantages of column chromatography over thin layer chromatography?
One is faster and more flexible, the other is a bit heavier
What are the advantages and limitations of column chromatography over thin layer chromatography?
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
What are the differences between column and thin layer chromatography?
In chromatographic terms, TLC has great advantages over the other chromatography modes, such as Liquid Chromatography (LC), Column Chromatography (CC), Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC).TLC's advantages are: (1) the ability to perform multiple analyses simultaneously; (2) speed and ease for scouting separation conditions, such as optimum solvent mixtures.
Disadvantages of using paper chromatography?
TLC has following advantages over paper chromatography: greater speed greater sensitivity for many substances than paper Small sample requirement Usually sharper preparation Different kind of reagents can be applied without damaging the plate
How is chromatography used to study plant pigment?
chromatography is basically a technique used for the separation of different components.... plant pigment consist of different components..... the sample is taken nd spotted over a chromatography paper..... nd den it is kept in suitable solvent to get separated...... different components travel different distance on chromatogram.... dis is how v cn use chromatography to study plant pigments...
How do you paint over drywall when the top layer of drywall has been peeled off?
If by top layer you mean the paper, then re-install drywall on that wall.
Why is chromatography used in the police?
the police use chromatography to solve crimes, for example if they wanted to solve if a drug or a substance was illegal or legal they would use paper chromatograhy which would mean they would place the substance or a drug on paper dip it in a testing liquid which could be water for example and if the paper turns purple for example that liquid may be illegal or if that dot of the certain substance rises over 5cm for example it may be illegal so chromatography comes in very handy for the police and solving there crimes ! Hope this helped ;) xxx
Why is ink never used for drawing the baseline on the chromatography paper?
Depending on the ink and the solvent used for the chromatography, the ink might also migrate and be separated into its components or smear over the paper. With the graphite of a pencil, this will not happen.
What is meant by tailing a spot in Chromatography?
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
What is the significance of TLC over paper chromatography?
Paper chromatography and TLC both use a solid stationary phase and liquid mobile phase. The only real difference is in the physical and chemical composition of the stationary phase. Paper chromatography uses a fibrous protein/polymer mesh (i.e. paper) as its stationary phase, TLC uses a compacted powder (usually silica or alumina) on a sealed backing.
What are disadvantages of paper?
It can threaten to give you a paper cut. Paper becomes weak over time so it has a lifetime limit. Normal paper is not waterproof. It is flammable It is easily damaged by water and insects. Not strong enough for heavy duty work.
How can one add texture to paper?
Adding texture to paper can be done using the wax resist method. A plain piece of paper is laid over an item such as sandpaper and rubbed with the side of a crayon. When a layer of water based paint is applied, the crayon will resist the paint and the paper will be left with a textured design.
