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In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
Fabiola Kshlerin
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∙ 9y agoIn a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
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What is the role of NaCl in DNA precipitation?
roll of Na CL in DNA extraction
Role of Cl in TE buffer solution?
TE buffer is a often used as a buffer solution in molecular biology, mainly in procedures involving DNA or RNA. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
What is the function of Tris cl in extraction of DNA?
TRIS maintains the pH of the solution. Basically it interacts with the lipopolysaccharides present on the outer membrane which helps to permeabilize the membrane. This effect is enhanced with the addition of EDTA.
Why does metabolic acidosis causes hypochloremia?
More Cl- is being excreted as Nh4Cl to buffer the excess acid in the renal tubules, leaving less Cl- in the Extracellular Fluid
How much Magnesium Carbonate is needed to buffer a solution of twenty litres with pH 2.75 consisting of Cl in water to a pH of 5.4?
Sorry, I mean a HCl solution in water, not Cl.
How do you prepare calcium buffer?
This might not be the best answer but, preparing a buffer solution allows one to keep the pH value the same when small amounts of acids or bases are added. Buffer solutions resist change in pH. Source: My Chemistry teacher's PowerPoint
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NH4+ + Cl- + K+ + OH- --> NH3 + H2O + Cl- + K+ Since the question is not asking for the net ionic equation, spectator ions should be included in the ionic equation as well.
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In solution, NaCl can split into Na+ and Cl- ions. These ions are indeed needed to stabilise the hydrophilic residues of the protein molecule that are exposed on the surface. So NaCl is a stabilising agent in various protocols even in the extraction, but it does not has any role in lysing the cells or neutralising other biomolecules.
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Cl-Cl is more covalent than H-Cl
What is the ion formula for chloride?
Cl- Cl-
How many electrons are the atoms sharing Cl-Cl?
In cl-cl bond 1 electron is sahred by each of Cl atom.
How many moles are there in 28.4 g of cholrine atom?
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