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Ribosomes start protein synthesis in the cytoplasm. Synthesis is completed either in the cytoplasm or in the lumen of the rough endoplasmic reticulum.

All proteins begin as polypeptides (chains of amino acids). The synthesis of a chain starts when a free (unattached) ribosome begins to move along a molecule of messenger RNA (mRNA) in the cytoplasm.

Once a small number of amino acids have been incorporated into the chain, large molecules in the cell "inspect" the chain. Certain sequences of amino acids are recognized as marking the chain for synthesis in the lumen of the rough endoplasmic reticulum (ER).

If the chain has one of these sequences, the mRNA and ribosome are moved to the ER and the ribosome is fixed in the ER membrane in such a way that the mRNA remains in the cytoplasm but the polypeptide chain is in the lumen of the ER. It is in the lumen that the chain is completed, and subsequently coils and folds to form the finished polypeptide.

If, on the other hand, the chain does not have one of these marker sequences, synthesis of the chain and its subsequent coiling and folding all take place in the cytoplasm.

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16y ago

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