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The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to split indole from the amino acid tryptophan. This division is performed by a chain of a number of different intracellular enzymes, a system generally referred to as "tryptophanase."
What else can I help you with?
Is staphylococcus epidermidis indole positive?
S.aureus is a positive gram. Indole test is a part the biochemistry test to determinate a bacteria negative gram. indole test used to determine S.aureus, the result is negative. That is improve which S.aureus haven't trytophanase enzym (please see indole test principle)bcb
Explain the chemical mechanism for detecting indole in a bacterial culture?
Indole is extracted from the medium and into the reagent layer (Kovac's) by the acidified butyl alcohol component and forms a complex with the p-dimethylaminobenzaldehyde, yielding the cherry red color seen when the culture is indole positive.
Indole positive gram negative bacteria rods?
Indole positive gram negative rod-shaped bacteria include members of the Enterobacteriaceae family such as Escherichia coli and Klebsiella pneumoniae. These bacteria produce indole from the amino acid tryptophan as a metabolic byproduct, which can be detected using the Kovac's reagent test. Indole production is a characteristic used in the identification and differentiation of these bacteria in the laboratory.
Is tryptophanase a exoenzyme?
No, tryptophanase is an endoenzyme, meaning it acts on a substrate within the cell where it is produced rather than outside the cell. It catalyzes the breakdown of tryptophan into indole, pyruvate, and ammonia.
Why is the secondary antibody used in an ELISA test conjugated with an enzyme?
The secondary antibody in an ELISA test is conjugated with an enzyme to amplify the signal produced when the antibody binds to the target antigen. This enzyme-substrate reaction generates a detectable signal that indicates the presence of the antigen, which allows for more sensitive and accurate detection in the ELISA assay.
What does the S.I.M. test for?
Sulfur Indole Motility test
Indole test in vibro cholera?
The indole positive test is used to test the absence or presence of the vibro cholera.
What test will differentiate between Proteus vulgaris and Citrobacter freundii?
Indole test,Proteus vulgaris is be indole positive and Citrobacter freundii will be indole negative
What are the indole test principles?
The indole test is based on the ability of certain bacteria to produce the enzyme tryptophanase, which converts tryptophan into indole. This indole is then detected by adding a reagent such as Kovac's reagent, which reacts with indole to produce a red color. A positive test indicates the presence of tryptophanase enzyme in the bacteria.
Is staphylococcus epidermidis indole positive?
S.aureus is a positive gram. Indole test is a part the biochemistry test to determinate a bacteria negative gram. indole test used to determine S.aureus, the result is negative. That is improve which S.aureus haven't trytophanase enzym (please see indole test principle)bcb
What are the results for a capsule stain for alcaligenes faecalis?
I dont have any answer, But i will post it when i will know . No change in color or reaction on Indole Test - Negative result on Indole Test.
Principle of ehrlich's test?
Ehrlich's test is a presumptive test for the presence of indole compounds, such as tryptophan, in a sample. It relies on the reaction between indole compounds and p-dimethylaminobenzaldehyde to produce a blue-colored complex. This test is commonly used in microbiology to detect the indole production by bacteria such as Escherichia coli.
What does kovac's reagent do in a reaction?
Used to test prescece of Indole in medium
What is a SIM tube?
A test that shows the sulfer, indole, and motility of a micro organism.
What test will differentiate between Klebsiella pneumonia and Citrobacter freundii?
Biochemical tests such as indole test and citrate utilization can help differentiate between Klebsiella pneumoniae and Citrobacter freundii. Klebsiella pneumoniae is indole negative and citrate positive, while Citrobacter freundii is indole positive and citrate negative. Additional tests like urease and motility can also aid in differentiation.
What is Staphylococcus aureus sim test results?
Staphylococcus aureus is negative for motility, and positive for the indole production. The SIM test tests for sulfur reduction, indole production and motility. Positive for indole production means tryptophan is broken down into indole and pyruvate and will give a red color. Motility, you will see the bacteria move outside of the stab. Positive for sulfur reduction will give a black color in the medium.
What are results of Imvic test on staphylococci?
Staphylococcus epidermidis indole test - negative methyl red - negative voges proskauer test - positive citrate test - no idea
