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The light is absorbed at a specific wavelength and this is because the electrons in the chemical bonds only absorb certain wavelengths of light. So, more concentration, more bonds and more electrons to absorb the light.

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If a solution is diluted will the absorbance decrease or increase?

If a solution is diluted, the absorbance will generally decrease. This is because the concentration of the absorbing species is lower in the diluted solution, resulting in fewer molecules to interact with the incident light and therefore lower absorbance.


I am determining reducing sugar by DNS method to optimize enzyme concentration for rice wine preparation.problem arose when absorbance increase then decrease when enzyme concentration increased.why di?

The decrease in absorbance after an initial increase in enzyme concentration during the DNS method may be due to substrate depletion, where the available sugars are rapidly consumed, leading to a reduction in the reaction rate. Additionally, excessive enzyme concentration can lead to enzyme denaturation or inhibition, which may further decrease the amount of reducing sugar produced. It's also possible that the reaction reached a saturation point, where adding more enzyme does not proportionally increase product formation, causing the observed decrease in absorbance.


Can the concentration of a substance change if it is an aqueous solution?

Yes. An aqueous solution means that at least one solute is dissolved in water. You can increase the concentration by adding more solute, or you can decrease the concentration by adding more water.


How can you change concentration?

To increase the concentration of a solution, you can decrease the amount of solvent by boiling off, or by evaporation, or you can simply add more solute. Conversely, to decrease the concentration, you can add more solvent.


What name is given to substance the decrease the concentartion of hydrogen ions in solution?

A substance that decreases the concentration of hydrogen ions in a solution is called a base or alkaline substance. It helps increase the pH of the solution, making it less acidic.


What happens to absorbance when volume is halved?

When the volume of a solution is halved, the concentration of the solute in that solution typically doubles, assuming no solute is lost and the total mass remains constant. According to Beer-Lambert Law, absorbance is directly proportional to concentration; thus, halving the volume will generally result in an increase in absorbance. This means that if you measure the absorbance after halving the volume, you would expect it to be higher than the original measurement.


Is Feedback inhibition means that an increase in a substance will decrease production of that substance?

Yes, feedback inhibition refers to a regulatory mechanism in which an increase in the concentration of a substance inhibits its own production. This typically occurs when the end product of a metabolic pathway binds to an enzyme involved in its synthesis, leading to a decrease in the activity of that enzyme. As a result, the overall production of that substance decreases, helping to maintain homeostasis within the cell.


Will the pH increase decrease or remain the same if the concentration of weak bases increases?

If the concentration of weak bases increases, the pH of the solution will increase. Weak bases accept protons in solution, leading to a decrease in hydrogen ion concentration and an increase in hydroxide ion concentration, which in turn raises the pH.


How do you change the concentration of a substance?

Add more or remove some of the substance from the mixture, or add or remove some other substance from the mixture. Removal is usually more difficult, with heating (distillation or boiling off) or filtration (of a solid) being among the most direct methods.


When citric acid is added will the pH increase or decrease?

When citric acid is added, or any acid for that matter, the pH of a substance will decrease. This is because since acids have a low pH they will decrease the pH of other substances when added to it.


How does temperature affect the volume of substance?

An increase in temperature will cause an increase in volume, while a decrease in temperature will cause a decrease in volume.


What increase absorbance of phenol in ethanol?

Possible factors that can increase the absorbance of phenol in ethanol are: increasing the concentration of phenol in the solution, using a higher path length cuvette for measurement, and selecting a wavelength for measurement where phenol has a higher molar absorptivity coefficient.