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Bacteria is stained to identify the organism and its structure. Staining also can help in identification of a species. Bacteria are translucent in appearance therefore a stain is applied to allow them to become visible.

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Do acid-fast bacteria stain gram-negative when subjected to the gram stain?

No, acid-fast bacteria do not stain gram-negative when subjected to the gram stain.


What is the gram stain of H5N1?

The Gram stain is used for bacteria and not for viruses.


Which stain sticks to the peptidoglycan in the cell wall of bacteria?

The stain that sticks to the peptidoglycan in the cell wall of bacteria is called crystal violet.


Is maneval's stain an acid stain?

Yes, Maneval's stain is an acid-fast stain used in microbiology to detect acid-fast bacteria such as Mycobacterium species. It involves using acid-alcohol to decolorize non-acid-fast bacteria while acid-fast bacteria retain the stain due to their waxy cell wall.


Why isn't the Gram stain used on acid-fast bacteria?

The Gram stain is not effective on acid-fast bacteria because their cell walls have a waxy layer that prevents the stain from penetrating.


Could a negatively charged dye stain a bacteria?

The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.


Does yogurt stain gram positive or gram negative?

Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.


What is the stain most useful in clinicians to decide for bacterial infection?

Methylene blue a basic stain is generally used to identify the external morphology of bacteria.The other stain which is used as differential stain and which can also differentiate the baceteia on the basis of their cell wall is gram stain i.e. Crystal voilet and is counter stained with Saffranine


Bacteria are divided into two groups based on how their cell walls absorb?

Bacteria are divided into two groups based on how their cell walls absorb the Gram stain. Gram-positive bacteria retain the stain due to their thick peptidoglycan cell wall, while Gram-negative bacteria do not retain the stain because of their thin peptidoglycan cell wall and an outer membrane.


Why does some bacteria stain purple and some red?

The purple stained bacteria are called gram positive bacteria and retain the stain after washing by having a thick cell wall one one layer of a heavily crossed linked material called peptidoglycan. ( Google that ) The red stained bacteria are gram negative because they posses two thinner layers of peptidogylcan cell wall that that do not hold the gram stain and it is mostly washed away in rinsing the bacterial samples.


Does the gram stain procedure work for all bacteria?

Yes. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive cells which do stain purple. In structural terms, the ability of a cell to become stained during the gram stain procedure is due to the chemical makeup of the cell wall.


Why is the counter stain for gram stain procedure differ from the counter stain used for acid fast procedure?

The counter stain used in the Gram stain procedure is typically safranin or basic fuchsin, which stains Gram-negative bacteria pink or red. In the acid-fast stain procedure, the counter stain used is typically methylene blue or brilliant green, which stains non-acid-fast bacteria blue or green, allowing acid-fast bacteria to retain the primary stain color (carbolfuchsin).