Microscopes

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As you open the diaphragm under the stage, you allow more light to pass through the specimen. This can improve depth of focus but, it can also cause the image to become more washed out.

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As you open the diaphragm under the stage, you allow more light to pass through the specimen. This can improve depth of focus but, it can also cause the image to become more washed out.

The function of the fine focusing mechanism in a microscope is to precisely adjust the focus of the specimen to achieve a clear and sharp image. It allows for small, incremental changes in the distance between the objective lens and the specimen, enabling detailed viewing and examination of the sample.

The rack and pinion substage in a microscope is used to adjust the height of the stage, allowing for fine focusing of the specimen. It enables precise positioning of the specimen in relation to the objective lens for clear viewing and imaging.

The inclination join connects the arm to the base of the microscope. The joint allows the microscope to be tilted for the convenience of the user.

Objective lenses are convex lenses.

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So you don't damage the lens by crushing the slide into it.

One limitation of electron microscopes is that they require specialized training to operate and may be more complex to use compared to light microscopes. Additionally, samples viewed with electron microscopes must be placed in a vacuum, which may affect the natural state of the specimen. Finally, electron microscopes are typically more expensive to purchase and maintain than light microscopes.

The field of view becomes narrower.

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Sometimes, if the stage is set high enough, the objective lens can hit or scratch the slide when it's changed, because each objective lens is a different length. From the back of the microscope, you cannot see if the lens is going to hit the slide or not, and run the risk of disturbing your sample. This problem can be prevented by looking from the side and stopping if it looks like the lens is going to hit the slide.

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An experiment can be use to gather informations because in this way we test different things to know if it is true or not and while discovering new things we can also gather informations from the given theme

Leeuwenhoek

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1590s

There are several type of microscopes, mainly, the one that we use in lab is a simple light microscope or a compound microscope. Then we have the phase contrast microscope, fluorescent microscope, electron microscope (transmission electron microscope [TEM] and scanning electron microscope [SEM]), confocal microscope and even dissection microscope the one which we use during dissections.

As the magnification of the objective increases, the FOV decreases

A compound microscope, like the common optical microscope is used to study objects too small to see with the naked eye. The difference between a common optical microscope, and a compound microscope is the number of objective lenses. A compound microscope will contain several lenses. This reduces distortion, and gives one multiple lenses with which to adjust magnification.

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