The transmission electron microscope operates on the same principle as the light microscope but uses electrons instead of light. What you can see with a light microscope is limited by the wavelength of light. Transmission electron microscopes use electrons as "light source" and their much lower wavelength makes it possible to get a resolution a thousand times better than with light microscope.
Hans Lippershey and (or) Zacharias Janssen, in Netherlands in 1590.
The media can not be altered it can only be destroyed.
A electron microscope uses beams of highly charged, energetic, electrons, due to these electrons this type of microscope can magnify the object it is looking at by two thousand times! So because we can magnify an object by such a great amount we can use electron microscopes to observe how atoms are arranged in an object and even look at the structure of an atom.
Advantages: You can see incredibly small particles with its intense magnifying power (up to about 20,000 times)
Disadvantages: Using EM's, you cannot view objects/organisms in live color, or in a live state (shooting thousands of electrons at incredibly high speeds just doesn't agree with living systems)
The Mechanical Parts are the objective lenses, Fine Adjustment, Coarse Adjustment, some of the microscopes can move the stage, and some microscopes can move the adjustable mirror...
Hope I can HELP!
Two knobs are used to get a clear image on low power. The coarse adjustment knob is used to make drastic changes to the image, and the fine adjustment knob is used to make small adjustments to the image. The reason two knobs are used is because you are supposed to get as clear an image as possible on low power before moving on to the next power, and same with medium power.
The price changes according to the type of microscope ; compound, electron, etc. It depends on how powerful it is. Believe it or not, an electron microscope can cost up to $150,00.00. However, you can get a compound microscope [ 10x , 30x , 50x ] for about $140.00.