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Can twins be created using the test tube method?

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yes...fraternal twins (different ova and different sperm). although it is also possible to generate identical twins I suppose (single ovum and single sperm). I have no idea what the statistics on this are however.
Although if you're *really* asking "can I *force* a fertilized ovum to split into two and generate a set of identical twins?" then I would have to say "Not likely".

Yes, an ovum can be forced to split. The above information is outdated.
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