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What does activity of an enzyme depend on?
The concentration of hydrogen ions in solution affects the enzyme activity. Each enzyme has maximal efficiency under an optimum pH. Since pH is one of the factors for the denaturation of proteins, if an enzyme is submitted to a pH level under which it is denatured there will be no enzymatic activity.
How might amino acid change affect the enzyme?
The pKA of enzyme affects its ionization which could alter enzyme activity. For pH < pKa, the value of vmax is constant and that for pH > pKa, vmax decreases; ie. enzyme activity starts to decline.
What is the true relationship between pH and enzyme action?
The enzyme has an optimal point of pH at which the enzyme works best. For example a catalase enzyme works best in a pH of 7. When the pH changes it denatures the enzyme causing it to not be able to react with the substrate.
What is varied when testing the effect of pH on enzyme activity?
pH
Temperature and can affect enzyme activity?
Ph level, enzyme concentration, substrate concentration etc
What is the effect of pH upon the relative enzyme activity?
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
If an enzyme is exposed to an unfavorable pH?
Enzymes shows different enzyme activity in different pH value.If the pH is high or low than the optimum pH there is a decrease in the activity of the enzyme.But if the pH is very high or very low it may lead to the deactivation of the enzyme.
What are factors affecting enzyme activity?
Concentration ( enzyme to substrate ), temperature and pH.
What conditions affects the function of enzymes?
The activity of an enzyme is affected by temperature, pH and the concentration of the substrate.
What are two factors that influence enzyme activity?
Concentration of the enzyme or it's substrate and the temperature.
What are the effects temperature and pH level have in the efficacy of digestive enzymes?
As you increase the temperature at first the activity of an enzymes will also increase, so the reaction will go faster. At a certain temperature, called the optimum temperature, the enzyme will work at its maximum rate. Above the optimum temperature the enzyme activity decreases. This is due to the loss of shape by the enzyme molecules, a process called denaturation. In mammals, most enzyme shave an optimum temperature of about 37 oC. Roughly speaking, the rate of an enzyme reaction doubles for each 10 oC rise in temperature, until the optimum temperature is reached. pH affects enzymes in a similar way. At a low pH many enzymes have a low activity. As the pH increases the enzyme activity increases until the optimum pH at which the enzyme has maximum activity. Above this pH enzyme activity decreases. These effects are also dueto denaturation of the enzyme molecules at extreme pH values. Many enzymes work best at around pH 7.0. An important exception is pepsin, a protein-digesting (protease) enzyme in the stomach. This works at low pH values such as those found in the stomach (around pH 2.0), due to the presence of hydrochloric acid.
What is the independent variable between temperature and enzyme activity?
To find out how enzyme concentration affects the activity of the enzyme you must:vary the concentration of the enzyme, by preparing different concentrations (keeping the volume of solution the same)keep the temperature, substrate concentration and pH constantmeasure the activity of the enzyme at each concentrationHow the enzyme activity is measured will depend on the specific enzyme involved.You need to have plenty of substrate (excess substrate) so it doesn't run out during the experiment.In this type of experiment, the enzyme activity is the dependent variable, the temperature, pH and substrate concentration are control variables and the enzyme concentration is the independent variable.
