0
How would Rf values changes of Cations if the developing chamber left uncovered in paper Chromatography?
Wiki User
∙ 11y ago
The solvent would keep on evaporating from the chromatogram and as a result it would keep on bringing the cations up, causing an increase in the Rf value.
Wiki User
∙ 11y ago
Wiki User
∙ 12y agoBecause the solution could evaporate
Add your answer:
Earn +20 pts
What is the purpose of the filter paper inside the developing chamber in chromatography?
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
Why do you need to saturate the chromatography chamber?
1) for uniform development 2) proper elution of sample
Why must the chromatography chamber be closed tightly?
so that the atmosphere in the jar becomes saturated with solvent.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
What would you use in a paper chromatography?
We use several things such as stationary phase which is a paper, mobile phase according to your solute and the mixture to be analysed. All these are placed in a closed chamber.
What is the purpose of the filter paper inside the developing chamber in chromatography?
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
Why must the chromatography chamber be closed tightly?
so that the atmosphere in the jar becomes saturated with solvent.
Why do you need to saturate the chromatography chamber?
1) for uniform development 2) proper elution of sample
What is the chamber that houses the developing fetus?
The womb (uterus).
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
Why is it important to cover the developing chamber in paper chromatography?
For thin layer or paper chromatography, this can be a very important step, especially if one or more of the components in the mobile phase have very high vapor pressures (or low boiling points). Without the equilibration, as the mobile phase starts to move up the plate, the more volatile components will evaporate into the air space above the mobile phase faster than the less volatile ones. This is change the composition of the mobile phase (not much, but sometimes enough to change the Rf's of the unknown spots and standards). If the mobile phase consists of only water or perhaps high boiling solvents, the equilibration step is not as important. If the mobile phase contains Methylene chloride, Diethyl ether or similar solvents, then the equilibration step can be very important in terms of reproducibility.
What would you use in a paper chromatography?
We use several things such as stationary phase which is a paper, mobile phase according to your solute and the mixture to be analysed. All these are placed in a closed chamber.
Why all burners must be turned off before any developing chambers?
Because some of the substances used in the chamber are flammable and toxic.
Is Iodine a soild or gas?
Elemental iodine is a solid. But it is a volatile solid in that chemists will use solid iodine in a chamber to "iodine stain" substances in thin layer chromatography. Thus iodine is like having a volatile solid.
How do you check the lining in an oil furnace combustion chamber?
Depending on the set up.... well simply unscrew all the bolts surrounding the burner itself(obviously depending on set up) you do not need to take the burner out if you are careful. remove this plate in my case it is cast iron and secured by five bolts. After removing it you will notice what looks very much like foam. this is incredibly fragile if you notice parts that are uncovered by this material or cracks you need to replace the lining. The combustion chamber is the lower chamber where the burner is inserted. If this helps...well great.
Was the subterranean chamber a fake chamber?
No the Subterranean chamber was a real chamber in the pyramid of Khufu.
Where to find unown in Pokemon LeafGreen?
Unown is a very common Pokemon that can be found in Dilford Chamber, Liptoo Chamber, Monean Chamber, Rixy Chamber, Scufib Chamber, Viapolis Chamber, and Weepth Chamber.
