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That all depends what you mean by "too long".

Potentially hazardous food, such as roast beef, should not be left out more than 2 hours. or one hour if the ambient temperature is around 90°F. At that time, the food should be refrigerated or re-heated - which would destroy vegetative cells.

Technically, you could heat the food to a temperature hot enough to kill any organisms, but this (called thermal death point) would also destroy the food. It is many, many times hotter than cooking temperature.

But just killing organisms isn't always the answer either - if the food has been left out "too long". Some bacteria and molds produce toxins that cannot be destroyed with normal heating methods. So the vegetative cells would be gone, but the toxin that could make someone sick would remain.

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Q: If roast beef has sat out on a table too long can it be reheated to reduce possibility of food poisoning?
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