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Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism.

Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is.

Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.

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Q: What is gene cloning and why do you need to clone a gene?
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Related questions

Is cloning an organism the same as cloning a gene?

no they are not same as the organism as cloning


Why is cloning an example of cloning?

Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.


Why should you use therapeutic cloning?

to clone a clincially important gene(to give protein)..eg:Insulin,glucocon,other factors


A noun from to clone?

to clone is to copy


Why is gene cloning known as a shotgun approach?

If you tray to clone something half the time it may as well be approching a shotgun, KABOOM! dead.


The cloning of many genes in hopes that at least one of the clonies of clones will contain the desired gene is referred to as the?

recombinant clone or chimeric DNA


What are the bad things about cloning?

It is believed that it will give the clone a shorter life span


Difference between cloning and gene transfer?

genetransferring is an engineering process where as cloning does not need genetical engineering whole


What is another term for gene?

This is also known as cloning (in Biotechnology) and differs from the common perception of a clone being a replica of the original parent, although the process of cloning is directed at producing such a replicate (which may be partial or total).


What plant cloning is applied?

clone


What are the main advantages and disadvantages of cloning?

The advantage of cloning is replacing or to clone organs for a specific need although it is illegal to clone an entire human body. The disadvantage is that cloning humans would be able to create custom built people by combining the possibilities in cloning and genetic engineering. The entire body will be the same and also it will effect population too.


What are facts about the IBM Clone?

The IBM PC Clone is the cloning a personal computer's generic.