It denatures them.
The value of high pH indicates the basicity of that substance.
"The environment" covers a lot of territory. Some places have higher Ph, some have lower. Mineral deposits, local industries, weather, etc. have an effect on Ph.
The pH of your pool should be ideally 7.4. this will change if you add chlorine as this will have the effect of raising pH which is why you need to control the pH with an Acid such as sodium bisulphate or marked up as pH minus (pH-) . Also when you add any water from the tap if the pH of incoming water is high then this will also change the pH. Watch out for low Total Alkalinity as this can cause pH bounce where you get fluctuating pH readings.
proteins
Because proteins are relatively insoluble at mid-range pH of around 4.5. The pH affects the charge of the protein causing a precipitate to for.
It denatures it.
Heating pure water the pH decrease.
The value of high pH indicates the basicity of that substance.
It is most effective at around pH 2, and becomes inactive over 5.
Temperature and enzymes. A good temperature example is the proteins in egg whites denaturing when exposed to heat. Specific enzymes denature specific proteins: lactase (an enzyme) denatures lactose (protein present in dairy products). Protein denaturation can be caused by a number of different factors. These include heat exposure, introduction to acidic surroundings, and exposure to high energy electromagnetic radiation.
When the intra and extra-cellular fluids become very acidic, proteins will not be broken down, because an acidosis bring the pH down, usually, no more than 1 pH. Therefore, that does not effect proteins much.
a change in shape of the active sites of the enzyme
Yes it can. Since enzymes are proteins, changes such as pH level, salinity, and even temperature can denature the proteins rendering them incapable of performing it's function because the shape has been altered.
Extreme heat, high or low pH, ionization of liquid medium, and a change in environment. The last one needs a little more explanation. If a protein is meant for a liquid-liquid environment, and it is brought into an air-liquid environment, it may denature. Proteins form because of the folds created from hydrophilic and hydrophobic folding. If there is no water in which these bonds form, the protein will denature.
Since most types of chlorine are very high in PH it may effect the over all PH of the water if it is not buffered.
Excessively high and low temperatures, or extreme pH's (high and low). The range of both varies greatly on the enzyme in question. High temperature and extreme pH can denature proteins readily. However other methods such as detergents and Salt Concentrations can also work.
Proteins are extremely sensitive to heat because the sudden rise of temperature breaks its hydrogen bonds. The sensitivity of the side chains and its three-dimensional structure also contributes to its reaction to extreme heat.