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Isothermal Titration Calorimetry (ITC) is a thermodynamic technique for monitoring any chemical reaction initiated by the addition of a binding component, and has become the method of choice for characterizing biomolecular interactions. When substances bind, heat is either generated or absorbed. Measurement of this heat allows accurate determination of binding constants (KB), reaction stoichiometry (n), enthalpy ( H) and entropy ( S), thereby providing a complete thermodynamic profile of the molecular interaction in a single experiment In ITC, a syringe containing a “ligand” solution is titrated into a cell containing a solution of the “macromolecule” at constant temperature. When ligand is injected into the cell, the two materials interact, and heat is released or absorbed in direct proportion to the amount of binding. As the macromolecule in the cell becomes saturated with ligand, the heat signal diminishes until only background heat of dilution is observed.
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On what principle does calorimetry depends?
law of conservation of energy.
What is the basic principle of biamperometric titration?
Basic principle of biamperometric titration is reduction-oxidation reaction which can produce electricity read by amperometer
What is the relationship between heat and calorimetry?
heat is a form of energy. where as "calorimetry" is the study of energy or study of heat. calorimetry not only deals with heat but also with other forms of energy.
What isothermal process?
Isothermal process is a process in which change in pressure and volume takes place at a constant temperature.
What are the type of conductometric titration?
types of conductometric titration: acid base titration complexometric titration replacement titration redox titration precipitation titration
What are the principles of calorimetry?
Isothermal Principle: total heat elimination = heat loss by radiation + convection + conduction + evaporation
Why isothermal titration calorimetry is is done with fluorescence quenching to study protein- small molecular inhibitor interaction?
quenching means (the act of extinguishing; causing to stop burning) "the extinction of the lights"
On what principle does calorimetry depends?
law of conservation of energy.
What is the basic principle of biamperometric titration?
Basic principle of biamperometric titration is reduction-oxidation reaction which can produce electricity read by amperometer
What are the deference of isothermal and non-isothermal crystallization kinetics study of the fats?
Isothermal crystallization done with respect to time and non isothermal with respect to temperature
Does calorimetry measure alcohol intake?
calorimetry=amount of calories you intake, not alcohol
What is a calorimetry?
A calorimetry is a wide headband which covers the ears, suitable for wearing on cold days.
What is mean by non isothermal columns?
isothermal means 'constant temperature' so to be non-isothermal means to have non-constant temperature.
What is principle involve in acid base titration?
it mainly involves to neutralisation of reaction and shows the rate of reaction
What is the relationship between heat and calorimetry?
heat is a form of energy. where as "calorimetry" is the study of energy or study of heat. calorimetry not only deals with heat but also with other forms of energy.
What is precipitation titration's principle?
Precipitation titrations are based upon reactions that yield ionic compounds of limited solubility.
What isothermal process?
Isothermal process is a process in which change in pressure and volume takes place at a constant temperature.
