Blank Sample in Spectrophotometry is used to measure the absorbance of light without sample. It is subtracted from the total absorbance for measurement of Absorbance from a sample's absorbance.

specific absorbance-

it is absorbance in a solution containing one gm of substance in 100 ml solvent in 1cm shell.

so it is having a difference with absorbance which is negative logarithm of incident light to the transmitted light.

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in primary light absorbed by outer molecule while in secondary re-absorbance occurs

Because when we read absorbance, it's the amount of light absorbed by the bacteria itself. Absorbance is directly related to the amount of bacteria. More absorbance = more bacteria.