In plasmid isolation RNA behaves as an unwanted material so to
separate it out RNAase is required which breaks down the RNA. This
is done to get pure quality of the product.
In plasmid isolation RNA behaves as an unwanted material so to
separate it out RNAase is required which breaks down the RNA. This
is done to get pure quality of the product.
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Most often, RNA is removed using the enzyme RNAase
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RNAase remove RNA from the solution as we need DNA only. RNAse
cuts down the RNA strand.
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Digestive enzymes break down nucleic acids:)
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RNA itself difficult to handle because of its unstable nature. Unlike DNA, RNA has free 2'-OH group in their ribose sugar that make them highly reactive. Other than this, RNAse contamination is everywhere (during isolation RNAase from our skin can kill RNA).