Results for: Agarose-gel-electrophoresis

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What is the process of agarose gel electrophoresis?

The method of gel electrophoresis is that it uses an electric curret passed through a gel medium to seperate nucleic acids of proteins. Small particles move easier than large (MORE)
In Biology

What is the role of TBE buffer in agarose gel electrophoresis?

TBE (Tris Borate EDTA) buffer acts as an electrolyte to carry  current through the gel, providing the motive force. Tris and  borate are buffer salts, for pH stabilization. (MORE)

Function of bromophenolblue in agarose gel electrophoresis?

Bromophenol blue or commasive blue functions as a sample staining dye or DNA staining dye it is mixed with sample before loading the sample in wells. The migration of bromophe (MORE)
In Science

Function of agarose in agarose gel electrophoresis?

Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to mak (MORE)
In Science

Why you have the range of concentration of agarose in gel electrophoresis?

The concentration of agarose will affect the density of the gel. A higher density will provide a greater number of obstacles, and therefore resistance, to the flow of DNA frag (MORE)

Why does DNA separate in agarose gel in electrophoresis?

On the DNA-sized scale, the agarose is porous and allows for DNA to move across it. The backbone of DNA is slightly negative, so when placed in the wells, the DNA is going to (MORE)