In the disk diffusion test (also called the Kirby Bauer test),
disks containing an antimicrobial agent are placed on the surface
of an agar plate containing a medium that has been inoculated with
the disease agent being tested, which will grow and fill the disk.
The antimicrobial agent diffuses into the medium, killing some of
the disease agent around where the anitmicrobial agent was
innoculated, depending on how susceptible the disease agent is to
the antimicrobial agent. The size of the area cleared of the
disease agent shows how effective the antimicrobial agent is.