how artificial chromosome are used as cloning vectors with example?

high

life was originated from three chromosome. three chromosome organism is the centromere of life. natural selection is much associated with gradual increase in chromosome due to effect of elcrtromagnetic radiation.

BAC = Bacterial Artificial Chromosome

-200-300Kb

-good for genomic libraries

-uses the bacterial F (sex) plasmid

-currently quite popular

YAC = Yeast Artificial Chromosome

-1Mb

-good for genomic libraries also

-difficult to manipulate and use

-less popular

there are many different vectores as:

1-plasmid system

2-bacteria phage lamda

3-cosmids

4-bacterio artificial system

5-puc system

the other cloning vectors are

m13 which is the oldest one.

and after the above all are:-

BAC(bacterial artificial chromosome)

YAC(yeat artificial chromosome)

TAC(transformation-competent artificial chromosome)