Density gradient is a spatial viration in density over an area

A density gradient is a tube filled with layers of two liquids mixed in varying proportions so that each layer has a different density. It is used by forensic scientists for soil characterization,

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Temperature and density are inversely proportional because increase in temperature increases the volume of a substance and thereby decreasing the density. In density gradient centrifugation, any change in temperature changes the sedimentation of a substance and therefore it may be in aqueous solution rather than pelleted or Vice verse

Density is simply the amount of "stuff" in a given amount of area.Density is how much mass a material has for a volume.