DNA sequence

This article does not cite any references or sources. Please help improve this article by adding citations to reliable sources. Unsourced material may be challenged and removed. (November 2007)

Electropherogram printout from automated sequencer for determining part of a DNA sequence

A DNA sequence or genetic sequence is a succession of letters representing the primary structure of a real or hypothetical DNA molecule or strand, with the capacity to carry information as described by the central dogma of molecular biology.

The possible letters are A, C, G, and T, representing the four nucleotide bases of a DNA strand — adenine, cytosine, guanine, thymine — covalently linked to a phosphodiester backbone. In the typical case, the sequences are printed abutting one another without gaps, as in the sequence AAAGTCTGAC, read left to right in the 5' to 3' direction. Short sequences of nucleotides are referred to as oligonucleotides and are used in a range of laboratory applications in molecular biology. With regard to biological function, a DNA sequence may be considered sense or antisense, and either coding or noncoding. DNA sequences can also contain "junk DNA."

Sequences can be derived from the biological raw material through a process called DNA sequencing.

In some special cases, letters besides A, T, C, and G are present in a sequence. These letters represent ambiguity. Of all the molecules sampled, there is more than one kind of nucleotide at that position. The rules of the International Union of Pure and Applied Chemistry (IUPAC) are as follows:^[1]

• A = adenine
• C = cytosine
• G = guanine
• T = thymine
• R = G A (purine)
• Y = T C (pyrimidine)
• K = G T (keto)
• M = A C (amino)
• S = G C (strong bonds)
• W = A T (weak bonds)
• B = G T C (all but A)
• D = G A T (all but C)
• H = A C T (all but G)
• V = G C A (all but T)
• N = A G C T (any)

See also

• DNA
• DNA sequencing
• DNA motif
• Full genome
• Single nucleotide polymorphism (SNP)
• Sequence analysis
• Polymerase chain reaction

References

External links

• A bibliography on features, patterns, correlations in DNA and protein texts